Department of Analytical Chemistry, Gdańsk University of Technology, 11/12 G. Narutowicza Street, 80-233, Gdańsk, Poland.
Department of Toxicology, Medical University of Lodz, 1 Muszyńskiego Street, 90-151, Lódź, Poland.
Appl Microbiol Biotechnol. 2022 Aug;106(13-16):5261-5272. doi: 10.1007/s00253-022-12043-5. Epub 2022 Jul 2.
The aim of the study was to develop an efficient method for the determination of monomethyl-mercury (MeHg) and total mercury (THg) content in materials such as fungal sporocarps and sclerotia. Certified Reference Materials (CRMs) with the assigned values of MeHg and THg as well as the control materials (dried mushrooms) with known content of THg were evaluated for method validation. Recovery of MeHg from reference materials was at the following levels: from tuna fish at 87.0 ± 2.3% (THg at 101.9 ± 1.2%), from fish protein at 99.4 ± 1.3% (THg at 92.70 ± 0.41%), and from dogfish liver at 96.45 ± 0.73%. Recovery of THg from the fungal control material CS-M-5 was at 104.01 ± 0.60% (contribution of MeHg in THg content was at 6.2%), from CS-M-4 at 101.1 ± 2.0% (contribution at 3.2%), from CS-M-3 at 100.55 ± 0.67% (contribution at 0.6%), and from CS-M-2 at 101.5 ± 2.7% (contribution at 3.7%). The content of MeHg in randomly selected wild fungi and their morphological parts was in the range from 0.006 to 0.173 mg kg dry weight (dw). In the case of THg, the concentration values were in the range from 0.0108 to 10.27 mg kg dw. The MeHg content in the control materials with the assigned THg values was determined. Since the control materials play an important role in all elements of the quality assurance system of measurement results, they can be used to analyse MeHg as the first control material for fungi. KEY POINTS: • An extraction procedure for MeHg analysis in fungi was developed and optimized. • Recovery of MeHg from the certified reference non-fungal materials was > 87%. • Fungal control materials with assigned THg concentration can serve also for MeHg analysis.
本研究旨在开发一种高效的方法，用于测定真菌子实体和菌核等材料中的单甲基汞（MeHg）和总汞（THg）含量。评估了具有 MeHg 和 THg 赋值的认证参考物质（CRM）以及具有已知 THg 含量的对照材料（干蘑菇），以验证方法的有效性。从金枪鱼中提取的 MeHg 回收率为 87.0 ± 2.3%（THg 为 101.9 ± 1.2%），从鱼蛋白中提取的 MeHg 回收率为 99.4 ± 1.3%（THg 为 92.70 ± 0.41%），从鲨鱼肝脏中提取的 MeHg 回收率为 96.45 ± 0.73%。从真菌对照材料 CS-M-5 中提取的 THg 回收率为 104.01 ± 0.60%（MeHg 在 THg 含量中的占比为 6.2%），从 CS-M-4 中提取的 THg 回收率为 101.1 ± 2.0%（占比为 3.2%），从 CS-M-3 中提取的 THg 回收率为 100.55 ± 0.67%（占比为 0.6%），从 CS-M-2 中提取的 THg 回收率为 101.5 ± 2.7%（占比为 3.7%）。随机选择的野生真菌及其形态部分的 MeHg 含量范围为 0.006 至 0.173 mg kg 干重（dw）。对于 THg，浓度值范围为 0.0108 至 10.27 mg kg dw。测定了具有指定 THg 值的对照材料中的 MeHg 含量。由于对照材料在测量结果质量保证体系的所有要素中都起着重要作用，因此它们可用于分析真菌中的 MeHg，作为真菌的第一个对照材料。关键点：• 开发并优化了真菌中 MeHg 分析的提取程序。• 从认证的非真菌材料中提取的 MeHg 回收率>87%。• 具有指定 THg 浓度的真菌对照材料也可用于 MeHg 分析。
