利用逆转录病毒转导的胎肝细胞生成造血谱系细胞特异性嵌合小鼠。

Generation of hematopoietic lineage cell-specific chimeric mice using retrovirus-transduced fetal liver cells.

机构信息

Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk 37673, Republic of Korea.

Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk 37673, Republic of Korea; Institute of Convergence Science, Yonsei University, Seoul 03722, Republic of Korea.

出版信息

STAR Protoc. 2022 Sep 16;3(3):101526. doi: 10.1016/j.xpro.2022.101526. Epub 2022 Jul 1.

DOI:10.1016/j.xpro.2022.101526

PMID:35779265

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9256946/

Abstract

Hematopoietic lineage cell-specific transgenic or knockout mice provide a valuable platform to identify the role of specific genes in hematopoiesis in vivo. Here, we describe protocols for preparation of retroviruses for overexpression or knockdown of a gene of interest, retroviral transduction of fetal liver cells, and generation of hematopoietic lineage cell-specific chimeric mice by transfer of the retrovirus-transduced fetal liver cells. This protocol is applicable for the study of in vivo functionality of a gene of interest in immune cells. For complete details on the use and execution of this protocol, please refer to Chang et al. (2013), Lee et al. (2016), and Hong et al. (2022).

摘要

造血谱系细胞特异性转基因或敲除小鼠为鉴定特定基因在体内造血中的作用提供了有价值的平台。这里，我们描述了用于过表达或敲低目的基因的逆转录病毒的制备、胎儿肝脏细胞的逆转录病毒转导以及通过转导的胎儿肝脏细胞转移生成造血谱系细胞特异性嵌合小鼠的方案。该方案适用于研究目的基因在免疫细胞中的体内功能。如需了解本方案的使用和执行的详细信息，请参考 Chang 等人（2013 年）、Lee 等人（2016 年）和 Hong 等人（2022 年）的研究。