生成人免疫系统小鼠的方案和用于分析人造血的流体力学注射。
Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis .
机构信息
MOE Key Laboratory of Model Animals for Disease Study, MOE Engineering Research Center of Protein and Peptide Medicine, The State Key Laboratory of Pharmaceutical Biotechnology, Chemistry and Biomedicine Innovation Center, Model Animal Research Center, Medical School of Nanjing University, Nanjing 210061, China.
Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
出版信息
STAR Protoc. 2022 Mar 3;3(1):101217. doi: 10.1016/j.xpro.2022.101217. eCollection 2022 Mar 18.
Human immune system (HIS) mice provide a valuable platform to investigate and modulate human hematopoiesis development . Here, we describe detailed protocols for the construction of HIS mice, modulation of human hematopoiesis using hydrodynamic injection of plasmids encoding cytokines of interest, and flow cytometry analysis of humanization levels and human immune subsets. This approach can be easily applied to screen or verify factors that regulate human hematopoiesis and immune system. For complete details on the use and execution of this protocol, please refer to Cardoso et al. (2021) and Li et al. (2017).
人免疫系统（HIS）小鼠为研究和调节人类造血发育提供了一个有价值的平台。在这里，我们描述了构建 HIS 小鼠、使用编码感兴趣细胞因子的质粒进行流体动力学注射调节人类造血、以及通过流式细胞术分析人类化水平和人类免疫亚群的详细方案。这种方法可以很容易地应用于筛选或验证调节人类造血和免疫系统的因素。有关该方案使用和执行的完整详细信息，请参阅 Cardoso 等人（2021 年）和 Li 等人（2017 年）。
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