Ishida Kazuki, Tanaka Katsunori, Kawakami Kei
Department of Bioscience, Graduate School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo, Japan.
Department of Biosciences, School of Biological and Environmental Sciences, Kwansei Gakuin University, Sanda, Hyogo, Japan.
MicroPubl Biol. 2022 Jun 10;2022. doi: 10.17912/micropub.biology.000586. eCollection 2022.
RNA polymerase I (Pol I) is a highly conserved complex that catalyzes the transcription of rRNA precursors in the nucleolus. In this study, we isolated a temperature-sensitive (ts) allele of Rpa2, the second largest subunit of Pol I in the fission yeast . We found that cells were severely defective in growth at temperatures above 32 °C. We also found that cells showed aberrant chromosome segregation and an abnormal ring-like nuclear structure at the restrictive temperature. These findings suggest that Rpa2 is essential for faithful nuclear division and nuclear structural organization in .
RNA聚合酶I（Pol I）是一种高度保守的复合物，可催化核仁中rRNA前体的转录。在本研究中，我们分离出了裂殖酵母中Pol I第二大亚基Rpa2的一个温度敏感（ts）等位基因。我们发现，细胞在32°C以上的温度下生长严重缺陷。我们还发现，细胞在限制温度下表现出异常的染色体分离和异常的环状核结构。这些发现表明，Rpa2对于裂殖酵母中准确的核分裂和核结构组织至关重要。
