Furukawa Rintaro, Hara Yuna, Furuya Keiko, Takahashi Kaho, Nishimura Rinka, Shingaki Tomoaki, Osada Hironari, Kondo Hirotaka, Ohmori Keitaro
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan.
Advanced Animal Medical Center, 1075 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-0934, Japan.
Vet Anim Sci. 2022 Jun 16;17:100256. doi: 10.1016/j.vas.2022.100256. eCollection 2022 Sep.
A pro-inflammatory role of interleukin (IL)-15 and IL-15 receptor (R) in chronic intestinal inflammation, such as inflammatory bowel disease, has been reported in humans. However, the contribution of IL-15 signaling in the pathogenesis of canine chronic enteropathy (CE) remains unclear. Therefore, as a first step in elucidating the importance of IL-15 signaling in canine CE, we measured the mRNA expression of and subunits, including , and , in the duodenal and colonic mucosae of healthy dogs and those with CE, including food-responsive enteropathy (FRE), antibiotic-responsive enteropathy (ARE), and immunosuppressant-responsive enteropathy (IRE). Real-time PCR analysis revealed significantly lower mRNA expression levels in the duodenal mucosa of dogs with IRE compared to healthy dogs. In contrast, the mRNA expression levels of , and in the duodenal mucosa and , and in the colonic mucosa did not differ among healthy dogs and those with FRE, ARE, or IRE. These findings suggest that decreased mRNA expression of might be involved in the pathogenesis of duodenitis in dogs with IRE. Moreover, even in canine CE, IL-15 signaling appears to play different roles in duodenitis and colitis in dogs with FRE, ARE, and IRE. However, there were no correlations between the gene expression levels of and clinical severity or histopathological scores in the duodenum of dogs with IRE. Further studies are necessary to investigate the IL-15Rα protein localization and to determine how impaired expression contributes to the development of duodenitis in dogs with IRE.
白细胞介素（IL）-15及其受体（R）在人类慢性肠道炎症（如炎症性肠病）中具有促炎作用。然而，IL-15信号传导在犬慢性肠病（CE）发病机制中的作用仍不清楚。因此，作为阐明IL-15信号传导在犬CE中重要性的第一步，我们测量了健康犬以及患有CE的犬（包括食物反应性肠病（FRE）、抗生素反应性肠病（ARE）和免疫抑制剂反应性肠病（IRE））十二指肠和结肠黏膜中IL-15Rα、β和γc亚基（包括IL-15Rα）的mRNA表达。实时PCR分析显示，与健康犬相比，患有IRE的犬十二指肠黏膜中IL-15Rα mRNA表达水平显著降低。相比之下，健康犬与患有FRE、ARE或IRE的犬之间，十二指肠黏膜中IL-15Rβ、γc和结肠黏膜中IL-15Rα、β、γc的mRNA表达水平没有差异。这些发现表明，IL-15Rα mRNA表达降低可能与患有IRE的犬十二指肠炎症的发病机制有关。此外，即使在犬CE中，IL-15信号传导在患有FRE、ARE和IRE的犬的十二指肠炎症和结肠炎中似乎也发挥着不同的作用。然而，患有IRE的犬十二指肠中IL-15Rα的基因表达水平与临床严重程度或组织病理学评分之间没有相关性。有必要进一步研究IL-15Rα蛋白的定位，并确定IL-15Rα表达受损如何导致患有IRE的犬十二指肠炎症的发展。
