Ogawa Misato, Osada Hironari, Hasegawa Ayana, Ohno Hikaru, Yanuma Nanako, Sasaki Kazuaki, Shimoda Minoru, Shirai Junsuke, Kondo Hirotaka, Ohmori Keitaro
Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
J Vet Intern Med. 2018 May;32(3):1019-1025. doi: 10.1111/jvim.15117. Epub 2018 Mar 23.
Mucosal imbalance of interleukin (IL)-1β and IL-1 receptor antagonist (Ra) has been reported in the duodenal mucosa of dogs with inflammatory bowel disease (IBD). However, the imbalance in the colonic mucosa and its role in duodenitis and colitis in IBD of dogs remain unclear.
To measure the expression of IL-1β and IL-1Ra proteins in the colonic mucosa of dogs with IBD, and to determine the effect of IL-1β on expression of occludin (ocln) mRNA, a tight junction component, in the duodenal and colonic mucosa of dogs with IBD.
Twelve dogs with IBD and 6 healthy dogs.
IL-1β and IL-1 Ra proteins in the colonic mucosa were quantified by ELISA in 7 of the 12 dogs with IBD. Expression of ocln mRNA in the duodenal and colonic mucosa was examined in the 12 dogs by real-time PCR.
The ratio of IL-1β to IL-1Ra in the colonic mucosa was significantly higher in dogs with IBD than in healthy dogs. The ex vivo experiment determined that IL-1β suppressed expression of ocln mRNA in the colonic mucosa, but not in the duodenal mucosa, of healthy dogs. Expression of ocln mRNA in the colonic mucosa, but not in the duodenal mucosa, was significantly lower in dogs with IBD than in healthy dogs.
A relative increase in IL-1β may attenuate ocln expression, leading to intestinal barrier dysfunction and promotion of intestinal inflammation in the colonic mucosa, but not in the duodenal mucosa, of dogs with IBD.
在患有炎症性肠病（IBD）的犬类十二指肠黏膜中，已报道白细胞介素（IL）-1β与IL-1受体拮抗剂（Ra）存在黏膜失衡。然而，结肠黏膜中的失衡情况及其在犬类IBD的十二指肠炎症和结肠炎中的作用仍不清楚。
测量患有IBD的犬类结肠黏膜中IL-1β和IL-1Ra蛋白的表达，并确定IL-1β对患有IBD的犬类十二指肠和结肠黏膜中紧密连接成分闭合蛋白（ocln）mRNA表达的影响。
12只患有IBD的犬和6只健康犬。
通过酶联免疫吸附测定法（ELISA）对12只患有IBD的犬中的7只的结肠黏膜中的IL-1β和IL-1 Ra蛋白进行定量。通过实时聚合酶链反应（PCR）检测12只犬的十二指肠和结肠黏膜中ocln mRNA的表达。
患有IBD的犬结肠黏膜中IL-1β与IL-1Ra的比值显著高于健康犬。体外实验确定，IL-1β抑制健康犬结肠黏膜而非十二指肠黏膜中ocln mRNA的表达。患有IBD的犬结肠黏膜而非十二指肠黏膜中ocln mRNA的表达显著低于健康犬。
IL-1β的相对增加可能会减弱ocln的表达，导致患有IBD的犬结肠黏膜而非十二指肠黏膜的肠屏障功能障碍并促进肠道炎症。
