Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Cell Cycle. 2022 Nov;21(21):2255-2267. doi: 10.1080/15384101.2022.2092816. Epub 2022 Jul 3.
The number and quality of oocytes in the ovarian reserve are related to fertility and reproductive lifespan in mammals. Some transcription factors have been demonstrated to determine oogenesis. The insulinoma-associated 2 () gene is a member of the Snail transcriptional repressor superfamily. Recent studies have demonstrated plays an essential role for insulin secretion and glucose intolerance in mice, but the functions of in reproduction remain elusive. Here, by examination of knockout mice, we found was essential for female fertility. Loss of resulted in female infertility with major defects in primordial follicle pool, ovarian folliculogenesis and ovulation. Transcriptomic profiling of ovaries suggests that loss of caused defects in oocyte meiosis and steroid synthesis. Both oocyte- and granulosa cell-expressed genes were dysregulated, including and other known genes involved in primary ovarian insufficiency. Together, these studies show that is required for oocyte development and their communication with ovarian somatic cells.
卵巢储备中的卵母细胞数量和质量与哺乳动物的生育能力和生殖寿命有关。一些转录因子已被证明可以决定卵母细胞的发生。胰岛素瘤相关 2 () 基因是 Snail 转录阻遏物超家族的成员。最近的研究表明， 在小鼠的胰岛素分泌和葡萄糖不耐受中发挥着重要作用，但 在生殖中的功能仍不清楚。在这里，通过对 基因敲除小鼠的研究，我们发现 对雌性生育能力至关重要。 缺失导致原始卵泡库、卵巢卵泡发生和排卵的主要缺陷，从而导致雌性不孕。对卵巢的转录组谱分析表明， 缺失导致卵母细胞减数分裂和类固醇合成缺陷。卵母细胞和颗粒细胞表达的基因均失调，包括 和其他已知参与原发性卵巢功能不全的基因。总之，这些研究表明 对于卵母细胞的发育及其与卵巢体细胞的通讯是必需的。
