Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Amsterdam University Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
Curr Stem Cell Res Ther. 2023;18(7):993-1000. doi: 10.2174/1574888X17666220630090418.
Repair of the nervous system in humans has always been complicated and faced difficulties. Cell transplantation approaches using biocompatible scaffolds might be an attractive therapeutic strategy for neuronal regeneration.
We designed a cell delivery platform based on polyurethane [PU] and modified it with iron oxide nanoparticles [FeO NPs] for neural induction of human-induced pluripotent stem cells [hiPSC]. Forskolin, IBMX, and different ratios of FBS were employed to induce neurogenesis of hiPSCs. Neural differentiations were assessed at the level of genes and proteins.
As was shown by MTT colorimetric assay, the proliferation and viability of SNL 76/7 on PU/ FeO were superior in comparison with pure PU and FeO. hiPSCs cultured with PU/FeO exhibited an elevated expression of β3-tubulin, MAP2, NSE, OLIG2, as compared to controls. Furthermore, Acridine Orange staining assured the survival and viability of hiPSCs after 14 days of differentiation.
All in all, our findings pointed out the biocompatibility and positive regulatory effect of PU/FeO on neural markers.
We believe this scaffold could be a potential candidate for future nerve differentiation applications.
人类神经系统的修复一直很复杂，并且面临困难。使用生物相容性支架的细胞移植方法可能是神经元再生的一种有吸引力的治疗策略。
我们设计了一种基于聚氨酯[PU]的细胞输送平台，并对其进行了氧化铁纳米粒子[FeO NPs]修饰，用于人诱导多能干细胞[hiPSC]的神经诱导。使用 forskolin、IBMX 和不同比例的 FBS 诱导 hiPSC 的神经发生。在基因和蛋白质水平评估神经分化。
正如 MTT 比色法所显示的那样，与纯 PU 和 FeO 相比，SNL 76/7 在 PU/FeO 上的增殖和活力更好。与对照组相比，在 PU/FeO 上培养的 hiPSCs 表达更高水平的β3-微管蛋白、MAP2、NSE、OLIG2。此外，吖啶橙染色确保了 hiPSC 在分化 14 天后的存活和活力。
总之，我们的研究结果表明了 PU/FeO 的生物相容性和对神经标记物的积极调节作用。
我们相信这种支架可能是未来神经分化应用的潜在候选者。
