Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, Republic of Korea.
Department of Bioactive Material Sciences and Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju, Republic of Korea.
Prion. 2022 Dec;16(1):84-90. doi: 10.1080/19336896.2022.2095186.
Prion diseases are fatal and irreversible neurodegenerative diseases induced by the pathogenic form of the prion protein (PrP), which is converted from the benign form of the prion protein (PrP). These diseases are characterized by an extended asymptomatic incubation period accompanied by continuous conversion of PrP to PrP. However, to date, the mechanism governing the conversion to PrP in the initial stages of prion disease has not been fully elucidated. We collected transcriptome data from the hippocampus of wild-type mice and prion-infected mice at 8 weeks post injection from the Gene Expression Omnibus and analysed differentially expressed genes and related signalling biological process using bioinformatic tools. We identified a total of 36 differentially expressed genes, including 22 upregulated genes and 14 downregulated genes. In addition, we identified that the cilium-related biological process was enriched in the early stages of prion disease. Furthermore, up- and down-regulated genes were associated with cilium-related cellular components and synapse-related cellular components, respectively. To the best of our knowledge, our study was the first to observe the upregulation of cilium-related genes in the early stages of prion disease.
朊病毒病是由朊病毒蛋白（PrP）的致病性形式引起的致命且不可逆转的神经退行性疾病，该蛋白由朊病毒蛋白（PrP）的良性形式转化而来。这些疾病的特征是潜伏期长且无症状，同时伴随着 PrP 向 PrP 的持续转化。然而，迄今为止，朊病毒病初期 PrP 转化的机制尚未完全阐明。我们从基因表达综合数据库中收集了野生型和感染朊病毒的小鼠在注射后 8 周的海马转录组数据，并使用生物信息学工具分析差异表达基因和相关信号生物学过程。我们共鉴定出 36 个差异表达基因，包括 22 个上调基因和 14 个下调基因。此外，我们还发现纤毛相关的生物学过程在朊病毒病的早期阶段富集。此外，上调和下调的基因分别与纤毛相关的细胞成分和突触相关的细胞成分相关。据我们所知，我们的研究首次观察到朊病毒病早期纤毛相关基因的上调。
