Department of General Surgery, The Second Affiliated Hospital of Xinjiang Medical University, Xinjiang, China.
Department of Gynecology, The Affiliated Tumor Hospital of Xinjiang Medical University, Xinjiang, China.
Cancer Biomark. 2022;35(1):71-81. doi: 10.3233/CBM-210213.
MicroRNAs (miRNAs) have been reported to play an important role in tumor progression by regulating the expression of target genes.
This study attempted to verify the role of miR-711 in gastric cancer (GC) progression by in vitro and in vivo assays.
The expression of miR-711 in tumor tissues and cells was detected by real-time quantitative PCR (qRT-PCR). Expression of MiR-711 in NCI-N87 and SNU-1 cells was detected by FISH. We transfected GC cells with miR-711 mimics or inhibitors. The effects of miR-711 on the proliferation and metastasis of GC cells were detected by CCK-8, wound healing and transwell assays. Dual-luciferase reporter gene assay was used to verify the targeting relationship between miR-711 and CD44. Xenograft assays was used to verify the regulatory effect of miR-711 on tumor growth.
In GC tissues and cell lines, the expression of miR-711 was down-regulated when compare with adjacent tissues or normal epithelial cells. The results indicated that overexpressing of miR-711 could suppress the GC cell proliferation, migration, and invasion through targeting CD44. The knockdown of CD44 showed similar effects as miR-711 overexpression in GC cells. Moreover, we confirmed these effects in the in vivo assays. Furthermore, we found that miR-711 could play a role by influencing tumor cell stemness.
MiR-711 plays vital roles as a tumor-suppressor by targeting CD44 and may be a therapeutic target for GC treatment.
microRNAs（miRNAs）已被报道通过调控靶基因的表达在肿瘤进展中发挥重要作用。
本研究通过体外和体内实验试图验证 miR-711 在胃癌（GC）进展中的作用。
实时定量 PCR（qRT-PCR）检测肿瘤组织和细胞中 miR-711 的表达。采用 FISH 检测 NCI-N87 和 SNU-1 细胞中 miR-711 的表达。转染 GC 细胞 miR-711 模拟物或抑制剂。CCK-8、划痕愈合和 Transwell 实验检测 miR-711 对 GC 细胞增殖和转移的影响。双荧光素酶报告基因实验验证 miR-711 与 CD44 的靶向关系。异种移植实验验证 miR-711 对肿瘤生长的调控作用。
与相邻组织或正常上皮细胞相比，GC 组织和细胞系中 miR-711 的表达下调。结果表明，过表达 miR-711 可通过靶向 CD44 抑制 GC 细胞增殖、迁移和侵袭。CD44 的敲低在 GC 细胞中表现出与 miR-711 过表达相似的效果。此外，我们在体内实验中证实了这些效果。此外，我们发现 miR-711 可以通过影响肿瘤细胞干性发挥作用。
miR-711 通过靶向 CD44 发挥重要的抑癌作用，可能成为 GC 治疗的一个治疗靶点。
