Berry E A, Trumpower B L
Anal Biochem. 1987 Feb 15;161(1):1-15. doi: 10.1016/0003-2697(87)90643-9.
Two procedures for analyzing overlapping optical spectra of mixtures of pyridine hemochromes are described, and extinction coefficients of pyridine hemochromes are provided for use with these methods. In the first procedure, absorbance is measured at a number of wavelengths equal to the number of components to be analyzed. This is the minimum amount of spectral data from which the concentration of each species can be calculated. In the second procedure, absorbance is measured at a number of wavelengths greater than the number of components to be analyzed. This redundancy of information makes it impossible to fit spectra which contain contributions from additional components, unless the spectra of the additional components are equal to linear combinations of the spectra of the species being analyzed. These two procedures are generally applicable to analyses of absolute or difference spectra of mixtures of components obeying Beer's law. The sensitivity to error in the absorbance measurements is only slightly greater than that for measuring a pure component at a single wavelength.
本文描述了两种分析吡啶血红素混合物重叠光谱的方法，并给出了吡啶血红素的消光系数，以便用于这些方法。在第一种方法中，在与待分析组分数目相等的多个波长处测量吸光度。这是能够计算每种物质浓度所需的最少光谱数据量。在第二种方法中，在大于待分析组分数目的多个波长处测量吸光度。这种信息冗余使得无法拟合包含其他组分贡献的光谱，除非其他组分的光谱等于被分析物质光谱的线性组合。这两种方法通常适用于对服从比尔定律的组分混合物的绝对光谱或差示光谱进行分析。吸光度测量中对误差的敏感度仅略高于在单一波长下测量纯组分时的敏感度。
