Effects of intraperitoneal injection of lipopolysaccharide-induced peripheral inflammation on dopamine neuron damage in rat midbrain.

INTRODUCTION

Current studies have documented neuroinflammation is implicated in Parkinson's disease. Recently, growing evidence indicated peripheral inflammation plays an important role in regulation of neuroinflammation and thus conferring protection against dopamine (DA) neuronal damage. However, the underlying mechanisms are not clearly illuminated.

METHODS

The effects of intraperitoneal injection of LPS (LPS )-induced peripheral inflammation on substantia nigra (SN) injection of LPS (LPS )-elicited DA neuronal damage in rat midbrain were investigated. Rats were intraperitoneally injected with LPS (0.5 mg/kg) daily for 4 consecutive days and then given single injection of LPS (8 μg) into SN with an interval of 0 (LPS 0 day ± LPS ), 30 (LPS 30 days ± LPS ), and 90 (LPS 90 days ± LPS ) days after LPS administration.

RESULTS

LPS increased the levels of inflammatory factors in peripheral blood in (LPS 0 day ± LPS ). Importantly, in (LPS 0 day ± LPS ) and (LPS 30 days ± LPS ), LPS attenuated LPS -induced DA neuronal loss in SN. Besides, LPS reduced LPS -induced microglia and astrocytes activation in SN. Furtherly, LPS reduced pro-inflammatory M1 microglia markers mRNA levels and increased anti-inflammatory M2 microglia markers mRNA levels. In addition, the increased T-cell marker expression and the decreased M1 microglia marker expression and more DA neuronal survival were discerned at the same area of rat midbrain in LPS -induced DA neuronal damage 30 days after LPS application.

CONCLUSION

This study suggested LPS -induced peripheral inflammation might cause T cells to infiltrate the brain to regulate microglia-mediated neuroinflammation, thereby protecting DA neurons.