Li Ruiqi, Du Fengjiao, Ou Songbang, Ouyang Nengyong, Wang Wenjun
Reproductive Medicine Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.
Reproductive Medicine Center, The First People's Hospital of Kashgar, Kashgar, People's Republic of China.
F S Rep. 2022 May 6;3(2):168-171. doi: 10.1016/j.xfre.2022.05.002. eCollection 2022 Jun.
To report a case of successful pregnancy involving embryos that wereaffected by bacterial contamination.
A case report.
Academic assisted reproductive center.
A 31-year-old infertile patient with obstructed fallopian tubes facing bacterial contamination in her embryos during in vitro fertilization.
The zona pellucida (ZP) of the embryos that was contaminated by bacteria was removed by acidic Tyrode's solution. The ZP-free embryos were then cultured in a time-lapse culture dish with 1 zygote per well until day 5 when a single ZP-free blastocyst was selected for transfer.
The rate of obtaining embryos without recurrence of bacterial contamination and the developmental potential of the embryos.
Twenty oocytes were retrieved and were coincubated with sperm in vitro overnight. A total of 9 zygotes with 2 pronuclei and 3 zygotes with 1 pronucleus were obtained. Unfortunately, all zygotes were contaminated by the bacteria. The ZP of 7 zygotes were removed using acidic Tyrode's solution (ZP-free group), whereas the remaining 5 zygotes and 3 metaphase II (MII) stage oocytes were washed with G-1 PLUS medium multiple times (washing treatment group). In the washing treatment group, all embryos experienced recontamination on day 2 and were dead by day 3. In the ZP-free group, 2 embryos were found to be recontaminated on day 2. The remaining 5 embryos that stayed uncontaminated were selected for blastocyst culture. On day 5, 2 of the cultured embryos developed into blastocysts. One blastocyst was transferred during the fresh cycle, and the other was vitrified. A single intrauterine gestation was confirmed 4 weeks after the transfer. At the time of writing this article, the patient was 30 weeks pregnant without any occurrence of intrauterine infection during pregnancy.
Zona pellucida removal is a safe and effective method to rescue embryos contaminated with bacteria.
报告一例成功妊娠病例，该妊娠涉及受细菌污染的胚胎。
病例报告。
学术性辅助生殖中心。
一名31岁的不育患者，输卵管阻塞，在体外受精期间其胚胎面临细菌污染。
用酸性台氏液去除被细菌污染的胚胎的透明带（ZP）。然后将无透明带胚胎培养在延时培养皿中，每孔1个受精卵，直到第5天，选择1个无透明带囊胚进行移植。
获得无细菌污染复发的胚胎的比率以及胚胎的发育潜能。
共获取20个卵母细胞，并与精子体外共孵育过夜。共获得9个具有2个原核的受精卵和3个具有1个原核的受精卵。不幸的是，所有受精卵均被细菌污染。使用酸性台氏液去除7个受精卵的透明带（无透明带组），而其余5个受精卵和3个中期II（MII）期卵母细胞用G-1 PLUS培养基多次洗涤（洗涤处理组）。在洗涤处理组中，所有胚胎在第2天再次受到污染，并在第3天死亡。在无透明带组中，2个胚胎在第2天被发现再次受到污染。选择其余5个未受污染的胚胎进行囊胚培养。在第5天，2个培养的胚胎发育成囊胚。1个囊胚在新鲜周期进行移植，另一个进行玻璃化冷冻保存。移植后4周确认单胎宫内妊娠。在撰写本文时，患者已怀孕30周，孕期未发生任何宫内感染。
去除透明带是挽救受细菌污染胚胎的一种安全有效的方法。
