Liu Hong-Lin, Cheng Cai, Zuo Ling-Zi, Yan Ming-Yue, He Yan-Lin, Huang Shi, Ke Ming-Jing, Guo Xiao-Liang, Feng Yu, Qian Hai-Feng, Feng Ling-Ling
Key Laboratory of Pesticide & Chemical Biology (CCNU), Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079 Hubei Province, China.
College of Environment, Zhejiang University of Technology, Hangzhou, 310014 Zhejiang Province, China.
iScience. 2022 Jun 16;25(7):104611. doi: 10.1016/j.isci.2022.104611. eCollection 2022 Jul 15.
Harmful blooms (HMBs) and microcystins (MCs) that are produced by seriously threaten water ecosystems and human health. This study demonstrates an eco-friendly strategy for simultaneous removal of MCs and HMBs by adopting unique hyperoxic graphene oxides (HGOs) as carrier and pure microcystinase A (PMlrA) as connecting bridge to form stable HGOs@MlrA composite. After oxidation, HGOs yield inherent structural strain effects for boosting the immobilization of MlrA by material characterization and density functional theory calculations. HGO exhibits higher loading capacities for crude MlrA (1,559 mg·g) and pure MlrA (1,659 mg·g). Moreover, the performances of HGO@MlrA composite, including the capability of removing MCs and HMBs, the ecological and human safety compared to MlrA or HGO treatment alone, have been studied. These results indicate that HGO can be used as a promising candidate material to effectively improve the application potential of MlrA in the simultaneous removal of MCs and HMBs.
由 产生的有害藻华（HMBs）和微囊藻毒素（MCs）严重威胁水生态系统和人类健康。本研究展示了一种生态友好型策略，即采用独特的高氧石墨烯氧化物（HGOs）作为载体，以纯微囊藻毒素酶A（PMlrA）作为连接桥，形成稳定的HGOs@MlrA复合材料，用于同时去除MCs和HMBs。氧化后，通过材料表征和密度泛函理论计算，HGOs产生内在结构应变效应，以促进MlrA的固定化。HGO对粗制MlrA（1,559 mg·g）和纯MlrA（1,659 mg·g）表现出更高的负载能力。此外，还研究了HGO@MlrA复合材料的性能，包括去除MCs和HMBs的能力、与单独的MlrA或HGO处理相比的生态和人类安全性。这些结果表明，HGO可作为一种有前景的候选材料，有效提高MlrA在同时去除MCs和HMBs方面的应用潜力。
