Pulliero A, Izzotti A, Pastorino L, Gandolfi S
Department of Health Sciences, University of Genoa, Via A. Pastore 1 Genoa, Italy.
UOC Mutagenesis and Cancer Prevention, IRCCS Ospedale Policlinico San Martino, Largo Rosanna Benzi, 10, Genoa, Italy.
Heliyon. 2022 Jun 19;8(6):e09753. doi: 10.1016/j.heliyon.2022.e09753. eCollection 2022 Jun.
Primary open angle glaucoma (POAG) is a progressive optic nerve degeneration, leading to irreversible visual damage. Alterations of the aqueous humor (AH), the biological fluid filling both the anterior and the posterior chambers of the eye, play a pathogenic role in POAG. AH protein composition is altered during glaucoma progression. Nestin protein was found to be differentially expressed in the AH of glaucomatous patients compared to unaffected matched controls.
Nestin was analyzed by an open quartz crystal microbalance (QCM) in the AH of 21 glaucomatous patients compared to nine unaffected controls. The surface of the electrode used in the QCM was coated with an analyte-specific recognition layer.
Positive nestin values were recorded in the AH collected from POAG patients; negative values of nestin detection were obtained by analyzing the AH collected from non-POAG glaucomatous patients and unaffected controls.
The present study proposes and validates a new clinically applicable approach to analyze biological markers in AH for POAG diagnosis.
原发性开角型青光眼（POAG）是一种进行性视神经退变，会导致不可逆的视力损害。房水（AH）是填充眼球前房和后房的生物流体，其改变在POAG中起致病作用。在青光眼进展过程中，房水蛋白质组成会发生改变。与未受影响的匹配对照组相比，巢蛋白在青光眼患者的房水中存在差异表达。
采用开放式石英晶体微天平（QCM）分析21例青光眼患者和9例未受影响对照组的房水中的巢蛋白。QCM中使用的电极表面涂有分析物特异性识别层。
从POAG患者收集的房水中记录到巢蛋白呈阳性值；通过分析从非POAG青光眼患者和未受影响对照组收集的房水，获得巢蛋白检测的负值。
本研究提出并验证了一种新的临床适用方法，用于分析房水中的生物标志物以诊断POAG。
