Department of Ophthalmology, Friedrich-Alexander-University of Erlangen-Nürnberg, Erlangen, Germany.
Department of Internal Medicine III, Institute of Clinical Immunology and Rheumatology, University of Erlangen-Nürnberg, Erlangen, Germany.
Front Immunol. 2021 May 5;12:550236. doi: 10.3389/fimmu.2021.550236. eCollection 2021.
Agonistic 2-adrenergic receptor autoantibodies (2-agAAbs) were recently observed in sera of patients with ocular hypertension (OHT), primary (POAG), and secondary open-angle glaucoma (SOAG), yet not in healthy controls (HCs). It was the aim of the present study to investigate the presence of 2-agAAb in aqueous humor (AH) samples of OAG patients and to correlate these with the corresponding 2-agAAb serum data.
Thirty-nine patients (21 male, 18 female) were recruited from the Department of Ophthalmology, University of Erlangen-Nürnberg: twenty-one POAG, 18 SOAG. Aqueous humor samples were collected during minimal invasive glaucoma surgery. Serum and AH samples were analyzed for 2-agAAb by a bioassay quantifying the beating rate of cultured cardiomyocyte (cut-off: 2 U).
Thirty-six of 39 (92.3%) and 34 of 39 (87.2%) of OAG patients showed a 2-agAAb in their sera and AH samples, respectively. All 2-agAAb AH-positive OAG patients were also seropositive. We also observed a 2-agAAb seropositivity in 95 and 89% of patients with POAG and SOAG, respectively. Beta2-agAAbs were seen in 86% (POAG) and 78% (SOAG) of AH samples. The 2-agAAb adrenergic activity was increased in the AH of patients with POAG (6.5 ± 1.5 U) when compared with those with SOAG (4.1 ± 1.1 U; p = 0.004). Serum 2-agAAb adrenergic activity did not differ between the cohorts [POAG (4.5 ± 1.5 U); SOAG (4.6 ± 2.1 U; p=0.458)]. No correlation of the beating rates were observed between serum and AH samples for group and subgroup analyses.
The detection of 2-agAAb in systemic and local circulations supports the hypothesis of a direct functional impact of these agAAbs on ocular G-protein coupled receptors. The high prevalence of 2-agAAb in serum and AH samples of patients with POAG or SOAG suggests a common role of these AAbs in the etiopathogenesis of glaucoma, independent of open-angle glaucoma subtype.
最近在患有眼高压（OHT）、原发性（POAG）和继发性开角型青光眼（SOAG）的患者血清中观察到激动性 2-肾上腺素能受体自身抗体（2-agAAb），但在健康对照者（HCs）中未观察到。本研究旨在检测 OAG 患者房水中是否存在 2-agAAb，并将其与相应的 2-agAAb 血清数据相关联。
从埃尔朗根-纽伦堡大学眼科系招募了 39 名患者（21 名男性，18 名女性）：21 名 POAG，18 名 SOAG。在微创青光眼手术期间采集房水样本。通过生物测定法分析血清和房水样本中的 2-agAAb，该方法定量培养的心肌细胞的跳动率（截止值：2 U）。
39 名 OAG 患者中有 36 名（92.3%）和 34 名（87.2%）在其血清和房水样本中分别显示出 2-agAAb。所有 2-agAAb 房水阳性的 OAG 患者均为血清阳性。我们还观察到 POAG 和 SOAG 患者的 2-agAAb 血清阳性率分别为 95%和 89%。在 POAG 患者的房水中可见 86%（POAG）和 78%（SOAG）的β2-agAAbs。POAG 患者的房水中的 2-agAAb 肾上腺素能活性（6.5 ± 1.5 U）高于 SOAG 患者（4.1 ± 1.1 U；p = 0.004）。POAG 患者（4.5 ± 1.5 U）和 SOAG 患者（4.6 ± 2.1 U；p = 0.458）之间的血清 2-agAAb 肾上腺素能活性无差异。组内和亚组分析均未观察到血清和房水样本之间的跳动率相关性。
在全身和局部循环中检测到 2-agAAb 支持这些 agAAb 对眼部 G 蛋白偶联受体具有直接功能影响的假说。POAG 或 SOAG 患者的血清和房水样本中 2-agAAb 的高患病率表明这些 AAbs 在青光眼的发病机制中具有共同作用，与开角型青光眼亚型无关。
Zotero 插件