Department of Radiology, Gyeongsang National University Hospital and College of Medicine, Gyeongsang National University, Jinju, Republic of Korea.
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Anticancer Res. 2022 Jul;42(7):3445-3452. doi: 10.21873/anticanres.15831.
BACKGROUND/AIM: Copine 1 (CPNE1) is a calciumdependent phospholipid protein that has been shown to regulate the AKT serine/threonine kinase 1 (AKT) signaling pathway to mediate its function in various cell types. However, little is known about the physiological function of this protein in breast cancer cells. We aimed to investigate the prognostic and therapeutic value of CPNE1 in erb-b2 receptor tyrosine kinase 2 [human epidermal growth factor receptor 2 (HER2)]-positive and luminal A subtypes of breast cancer.
Western blotting, cell viability, wound-healing and invasion assays were performed on SK-BR3 and MCF-7 breast cancer cells with forced overexpression of CPNE1. CPNE1 immunohistochemical (IHC) staining and bioinformatics analysis were performed on specimens from patients with breast cancer and compared to normal breast samples.
CPNE1 overexpression promoted AKT activation, and increased cell viability and cell motility in SK-BR3 and MCF-7 breast cancer cells. In addition, invasive capabilities of SK-BR3 cells were increased by the overexpression of CPNE1. The expression levels of CPNE1 were higher in HER2-positive and luminal A subtypes of human breast cancer tissues compared with those in adjacent normal tissues. Furthermore, CPNE1 expression was increased in RNA microarray analysis of samples from patients with breast cancer compared to normal breast samples.
CPNE1 may play a key role in the pathophysiology of HER2-positive and luminal A subtypes of breast cancer.
背景/目的：Copine 1（CPNE1）是一种钙依赖性磷脂蛋白，已被证明可调节 AKT 丝氨酸/苏氨酸激酶 1（AKT）信号通路，以调节其在各种细胞类型中的功能。然而，关于这种蛋白质在乳腺癌细胞中的生理功能知之甚少。我们旨在研究 CPNE1 在表皮生长因子受体 2（HER2）阳性和管腔 A 型乳腺癌中的预后和治疗价值。
在 SK-BR3 和 MCF-7 乳腺癌细胞中通过强制过表达 CPNE1 进行 Western 印迹、细胞活力、划痕愈合和侵袭测定。对乳腺癌患者的 CPNE1 免疫组织化学（IHC）染色和生物信息学分析，并与正常乳腺样本进行比较。
CPNE1 过表达促进 AKT 激活，并增加 SK-BR3 和 MCF-7 乳腺癌细胞的细胞活力和细胞迁移。此外，CPNE1 的过表达增加了 SK-BR3 细胞的侵袭能力。与相邻正常组织相比，人乳腺癌组织中 HER2 阳性和管腔 A 型中 CPNE1 的表达水平更高。此外，与正常乳腺样本相比，从乳腺癌患者样本的 RNA 微阵列分析中也增加了 CPNE1 的表达。
CPNE1 可能在 HER2 阳性和管腔 A 型乳腺癌的病理生理学中起关键作用。
