Kuruoglu Tuba, Altun Gamze, Kuruoglu Enis, Turan Derya Bayırlı, Önger Mehmet Emin
Department of Infectious Diseases and Clinical Microbiology, Medical Faculty, Ondokuz Mayıs University, Samsun, Turkey.
Department of Histology and Embryology, Medical Faculty, Ondokuz Mayıs University, 55139, Samsun, Turkey.
Chin Neurosurg J. 2022 Jul 5;8(1):15. doi: 10.1186/s41016-022-00284-2.
Shunt systems are used to provide cerebrospinal fluid drainage in the treatment of hydrocephalus. Recently, antibiotic-impregnated shunt systems are used to prevent colonization in the ventriculoperitoneal catheters. Methicillin-resistant Staphylococcus aureus (MRSA) is the most common causative microorganism of shunt infections. The aim of the study is to investigate effects of several substances on MRSA biofilms in the ventriculoperitoneal catheters.
The present study consists of mainly eight groups (each has two subgroups as antibiotic-impregnated and nonantibiotic-impregnated catheters). In addition, each group contains six molds using MRSA strains. In this study, daptomycin (DAPT) (2 mg/ml), vancomycin (VAN) (10 mg/ml), linezolid (LIN) (2 mg/ml), N-acetylcysteine (NAC) (6 mg/ml), and various combinations of these substances were used to evaluate the treatment against MRSA using scanning electron microscope (SEM) images and microbiological enumeration.
The colony count in the antibiotic-impregnated samples significantly decreased compared to nonantibiotic-impregnated samples in the MRSA, MRSA + DAPT, and MRSA + LIN groups (p < 0.01), respectively. Conversely, the colony count in antibiotic-impregnated samples significantly increased compared to nonantibiotic-impregnated samples in NAC + DAPT and NAC + VAN groups (p < 0.01), respectively.
The results showed that the use of antibiotic-impregnated catheters has a significant impact on the prevention of infection whereas the combination of NAC and DAPT showed better antibiofilm and antibacterial effects than other combinations on the prevention and treatment of nonantibiotic-impregnated catheter infections.
分流系统用于在脑积水治疗中提供脑脊液引流。近来，抗生素浸渍分流系统用于预防脑室腹腔导管的定植。耐甲氧西林金黄色葡萄球菌（MRSA）是分流感染最常见的致病微生物。本研究的目的是调查几种物质对脑室腹腔导管中MRSA生物膜的影响。
本研究主要包括八组（每组有两个亚组，分别为抗生素浸渍导管和非抗生素浸渍导管）。此外，每组包含六个使用MRSA菌株的模型。在本研究中，使用达托霉素（DAPT）（2mg/ml）、万古霉素（VAN）（10mg/ml）、利奈唑胺（LIN）（2mg/ml）、N-乙酰半胱氨酸（NAC）（6mg/ml）以及这些物质的各种组合，通过扫描电子显微镜（SEM）图像和微生物计数来评估对MRSA的治疗效果。
在MRSA、MRSA + DAPT和MRSA + LIN组中，抗生素浸渍样本中的菌落计数与非抗生素浸渍样本相比分别显著降低（p < 0.01）。相反，在NAC + DAPT和NAC + VAN组中，抗生素浸渍样本中的菌落计数与非抗生素浸渍样本相比分别显著增加（p < 0.01）。
结果表明，使用抗生素浸渍导管对预防感染有显著影响，而NAC和DAPT的组合在预防和治疗非抗生素浸渍导管感染方面比其他组合表现出更好的抗生物膜和抗菌效果。
