经 PCR 检测证实，用感染猫弓首蛔虫的 BALB/c 小鼠进行幼虫移行症实验。
Larva migrans in BALB/c mice experimentally infected with Toxocara cati ensured by PCR assay.
机构信息
Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, P.O. Box: 3149779453, Karaj, Iran.
出版信息
BMC Vet Res. 2022 Jul 5;18(1):257. doi: 10.1186/s12917-022-03366-6.
BACKGROUND
Toxocara cati, the cat roundworm, is a parasitic nematode that known to cause toxocariasis in intermediate hosts and humans. In this study, we characterized the dynamics of T. cati larvae migration in BALB/c mice after inoculation with eggs and ensured the migration detecting the larval DNA by a PCR. To evaluate the dynamics of larval migration and distribution, twenty-four BALB/c mice were orally inoculated with 2500 T. cati infective eggs and the visceral organs of the infected animals were examined by pepsin digestion and microscopic parasite counts, followed by PCR at day 1 to 28 post-inoculation.
RESULTS
The PCR assays were successfully used for detection of T. cati larvae in tissue samples and T. cati larvae and the DNAs were found in the liver, lungs, heart, kidneys and the brain. We detected T. cati in 92.2% of tissue samples by PCR, 30% higher than the conventional pepsin digestion technique.
CONCLUSION
Our findings demonstrated that the PCR assay is a sensitive and specific for the detection of T. cati larvae. Therefore, it could become a useful tool for the investigation of the dynamics of larval migration and Toxocara infection in murine model.
背景
猫蛔虫，猫的圆线虫，是一种寄生性线虫，已知会在中间宿主和人类中引起弓蛔虫病。在这项研究中，我们描述了接种卵后猫蛔虫幼虫在 BALB/c 小鼠体内的迁移动态，并通过 PCR 确保了幼虫 DNA 的检测。为了评估幼虫迁移和分布的动态，将 24 只 BALB/c 小鼠经口接种 2500 条感染性猫蛔虫卵，并在接种后第 1 至 28 天通过胃蛋白酶消化和寄生虫计数显微镜检查来检查感染动物的内脏器官，随后进行 PCR 检测。
结果
PCR 检测方法成功地用于检测组织样本中的猫蛔虫幼虫，并且在肝脏、肺部、心脏、肾脏和大脑中均发现了猫蛔虫幼虫和 DNA。通过 PCR 检测到 92.2%的组织样本中有猫蛔虫，比传统的胃蛋白酶消化技术高 30%。
结论
我们的研究结果表明，PCR 检测法对猫蛔虫幼虫的检测具有敏感性和特异性。因此，它可能成为研究鼠模型中幼虫迁移和弓蛔虫感染动态的有用工具。
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