French Adrienne F, Castillo-Alcala Fernanda, Gedye Kristene R, Roe Wendi D, Gartrell Brett D
School of Veterinary Science, Massey University, Private Bag 11-222, Palmerston North, New Zealand.
Int J Parasitol Parasites Wildl. 2020 Feb 24;11:221-228. doi: 10.1016/j.ijppaw.2020.02.011. eCollection 2020 Apr.
Sporadic cases of visceral and neural nematode have been diagnosed at necropsy in the endangered New Zealand kiwi ( spp.), but the causative organisms have not yet been definitively identified. From an initial group of five affected kiwi, PCR was performed on DNA extracted from archival formalin-fixed paraffin-embedded tissue sections in which larval nematodes had been histologically identified. Sequencing of positive results from four out of the five kiwi aligned with sequences from , a nematode parasite whose definitive host is the domestic cat. PCR was then performed on a second group of 12 kiwi that had histologic inflammatory lesions consistent with , but variable larval presence. Repeatable positive PCR results were only achieved in one tissue, in which larval organisms were histologically confirmed. This study supports the use of PCR as an alternative or adjunct to the morphological identification of nematode larvae in formalin-fixed histopathological samples, as well as showing that in investigation of , PCR has greatest chance of success from sections where nematode larvae are evident histologically. The identification of from lesions of in kiwi reflects an indirect, parasite-mediated effect of an invasive mammalian species on a native species.
在濒危的新西兰几维鸟(几维属)尸检时已诊断出散发性内脏和神经线虫病病例,但致病生物尚未得到明确鉴定。从最初的五只患病几维鸟开始,对从存档的福尔马林固定石蜡包埋组织切片中提取的DNA进行了聚合酶链反应(PCR),这些组织切片中已通过组织学方法鉴定出幼虫线虫。五只几维鸟中有四只的阳性结果测序与一种线虫寄生虫的序列一致,该线虫的终末宿主是家猫。然后对第二组12只几维鸟进行了PCR,这些几维鸟有与线虫病一致但幼虫存在情况各异的组织学炎症病变。仅在一个组织中获得了可重复的阳性PCR结果,其中通过组织学方法确认存在幼虫生物。这项研究支持将PCR用作福尔马林固定组织病理学样本中线虫幼虫形态学鉴定的替代方法或辅助方法,同时还表明,在调查线虫病时,从线虫幼虫在组织学上明显可见的切片中进行PCR成功的机会最大。在几维鸟的线虫病病变中鉴定出该寄生虫反映了一种入侵哺乳动物物种对本地物种的间接的、由寄生虫介导的影响。