Biomedical Science Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Environmental Health and Industrial Safety Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Pak J Pharm Sci. 2022 May;35(3):865-872.
Four new organotin(IV) dithiocarbamate compounds with general formulae of PhnSn [SCN(CHCHOCHCH)] for compound 1 and 2; and PhSn[SCN(CH)(CHCHCH)] for compound 3 and 4 were successfully synthesized via in situ insertion method. These compounds namely, diphenyltin(IV)- [1] and triphenyltin(IV) N,N-bis(2-ethoxyethyl)dithiocarbamate [2], diphenyltin(IV)- [3] and triphenyltin(IV) N-methyl-N-phenethyldithiocarbamate [4] were each characterized with CHNS elemental analysis, FT-IR and NMR spectroscopies (H, C and Sn). The compounds were then assessed for their cytotoxicity against K562 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazholium bromide (MTT) assay upon 24 h treatment. All compounds produced the essential IR absorption bands and displayed important NCS peak in C NMR spectroscopy. From the cytotoxicity studies using MTT assay, the compounds were shown to inhibit cell proliferation in K562 leukemic cells with IC values ranging from 1.48 to 4.52 μM, and in the manners more cytotoxic compared to standard used imatinib.
四种新的有机锡（IV）二硫代氨基甲酸盐化合物，通式为 PhnSn [SCN(CHCHOCHCH)]，用于化合物 1 和 2；PhSn[SCN(CH)(CHCHCH)]，用于化合物 3 和 4，通过原位插入法成功合成。这些化合物，即二苯基锡（IV）-[1]和三苯基锡（IV）N，N-双（2-乙氧基乙基）二硫代氨基甲酸盐[2]、二苯基锡（IV）-[3]和三苯基锡（IV）N-甲基-N-苯乙基亚磺酰胺[4]，均通过 CHNS 元素分析、FT-IR 和 NMR 光谱（H、C 和 Sn）进行了表征。然后，在 24 小时处理后，使用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐（MTT）测定法评估了这些化合物对 K562 细胞的细胞毒性。所有化合物均产生了基本的 IR 吸收带，并在 C NMR 光谱中显示出重要的 NCS 峰。通过 MTT 测定法进行的细胞毒性研究表明，这些化合物在 K562 白血病细胞中抑制细胞增殖，IC 值范围为 1.48 至 4.52 μM，与标准使用的伊马替尼相比，具有更强的细胞毒性。
