Program of Biomedical Science, Center for Toxicology and Health Risk Study, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
Program of Biomedical Science, Centre for Healthy Ageing and Wellness, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
Anticancer Agents Med Chem. 2024;24(1):58-65. doi: 10.2174/0118715206266851231025054446.
Continuous research for new effective drugs to treat cancer has improved our understanding on the mechanism of action of these drugs and paved new potential for their application in cancer treatments. In this study, organotin compounds known as triphenyltin ethyl phenyl dithiocarbamate and triphenyltin butyl phenyl dithiocarbamate were investigated for their toxicity on leukemia cell line (K562) and non-cancerous cell line (Chang liver cell and lung fibroblast, V79 cell).
MTT assay was performed to evaluate the cytotoxic effects of both compounds toward the cells after 24, 48 and 72 hours of exposure or treatment. The alkaline comet assay was conducted to determine the DNA damage on K562 cells after been exposed to both compounds for 30, 60 and 90 minutes.
The IC values obtained from K562 cells ranged from 0.01 to 0.30 μM, whereas for both Chang liver cell and lung fibroblast V79 cell, the values ranged from 0.10 to 0.40 μM. For genotoxicity evaluation, the percentage of damaged DNA is measured as an average of tail moment, and was found to be within 1.20 to 2.20 A.U while the percentage of DNA intensity ranging from 1.50 to 3.50% indicating no genotoxic effects.
Both compounds are cytotoxic toward leukemia cells and non-cancerous cells but do not exert their genotoxic effects towards leukemia cell.
为了寻找新的有效抗癌药物，科学界进行了持续不断的研究。这些研究增进了我们对这些药物作用机制的理解，并为它们在癌症治疗中的应用开辟了新的潜力。在这项研究中，研究了两种有机锡化合物，即三苯基锡乙基苯基二硫代氨基甲酸酯和三苯基锡丁基苯基二硫代氨基甲酸酯，以研究它们对白血病细胞系（K562）和非癌细胞系（Chang 肝细胞和肺成纤维细胞，V79 细胞）的毒性。
使用 MTT assay 评估了这两种化合物在暴露或处理 24、48 和 72 小时后对细胞的细胞毒性作用。使用碱性彗星试验来确定 K562 细胞在暴露于两种化合物 30、60 和 90 分钟后的 DNA 损伤情况。
从 K562 细胞中获得的 IC 值范围从 0.01 到 0.30 μM，而对于 Chang 肝细胞和肺成纤维细胞 V79 细胞，IC 值范围从 0.10 到 0.40 μM。对于遗传毒性评估，损伤 DNA 的百分比测量为尾部矩的平均值，发现其在 1.20 至 2.20 A.U 之间，而 DNA 强度的百分比范围从 1.50 到 3.50%，表明没有遗传毒性作用。
这两种化合物对白血病细胞和非癌细胞均具有细胞毒性，但对白血病细胞没有遗传毒性作用。
