State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, China.
Medical College, Guangxi University, Nanning 530004, China.
Dalton Trans. 2022 Jul 26;51(29):11005-11012. doi: 10.1039/d2dt01393b.
Three fluorescent glycosyl-rhodamine probes with good selectivity and sensitivity toward Hg were developed. The detection limit of the probes toward Hg is as low as 94.6 nM, which can be used to detect trace Hg in solution. 1 : 1 stoichiometry was the most possible recognition mode of the probes toward Hg, and the OFF/ON mechanism of the probes toward Hg could be attributed to the closing or opening of the rhodamine spiral structure caused by Hg. The detection of Hg is reversible, which is beneficial for the recycling of probes. Moreover, these low cytotoxic probes can be safely and selectively applied to monitor Hg levels in hepatocytes, and the fluorescence response follows a trend of Rho-Gal > Rho-Lac > Rho-Glu in HepG2 cells because the galactose group in Rho-Gal can selectively recognize ASGPR overexpressed on HepG2 cells.
开发了三种对 Hg 具有良好选择性和灵敏度的荧光糖基罗丹明探针。这些探针对 Hg 的检测限低至 94.6 nM，可用于检测溶液中的痕量 Hg。探针与 Hg 的最可能的识别模式为 1:1 计量比，探针与 Hg 的 OFF/ON 机制可归因于 Hg 引起的罗丹明螺旋结构的闭合或打开。Hg 的检测是可逆的，这有利于探针的回收。此外，这些低细胞毒性的探针可以安全且选择性地应用于监测肝细胞中的 Hg 水平，并且荧光响应遵循 Rho-Gal>Rho-Lac>Rho-Glu 的趋势，因为 Rho-Gal 中的半乳糖基可以选择性地识别在 HepG2 细胞上过表达的 ASGPR。
