State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
Anal Biochem. 2022 Oct 1;654:114800. doi: 10.1016/j.ab.2022.114800. Epub 2022 Jul 2.
Sulfite (SO) is considered as a monitor of a wide range of physiological processes. However, cells and tissues are adversely affected when the body ingests high level of sulfite. Here, we designed and synthesized a "turn on" fluorescent probe ImiSft-1 with 2-cyano-N-methylacetamide as the specific recognition site of SO. This probe predominantly achieved high response intensity to SO and desirable properties such as large Stokes shift (∼180 nm), fast response time (within 15 s), and high sensitivity (LOD = 0.12 μM). Importantly, the probe was highly selective for sulfite from other bio-species including biological thiols. Other functional properties included broad pH adaptability (5.0-10.0) and low cytotoxicity. Given these advantages and the fluorescence imaging in living MCF-7 cells, it was demonstrated that probe ImiSft-1 could monitor the changes of sulfite concentration in living cells.
亚硫酸盐 (SO) 被认为是广泛生理过程的监测物。然而，当人体摄入高水平的亚硫酸盐时，细胞和组织会受到不良影响。在这里，我们设计并合成了一种“开启”型荧光探针 ImiSft-1，其以 2-氰基-N-甲基乙酰胺作为 SO 的特异性识别位点。该探针主要对 SO 表现出高响应强度和理想的性质，例如大斯托克斯位移（约 180nm）、快速响应时间（在 15 秒内）和高灵敏度（LOD=0.12μM）。重要的是，该探针对包括生物硫醇在内的其他生物物种的亚硫酸盐具有高度选择性。其他功能特性包括较宽的 pH 适应性（5.0-10.0）和低细胞毒性。鉴于这些优势以及在活 MCF-7 细胞中的荧光成像，证明探针 ImiSft-1 可以监测活细胞中亚硫酸盐浓度的变化。
