College of Agronomy, Northwest A&F University, Yangling, 712100 Shaanxi, China.
Gene. 2022 Sep 5;838:146707. doi: 10.1016/j.gene.2022.146707. Epub 2022 Jul 2.
An increasing number of studies have shown that long non-coding RNAs (lncRNAs) play an important role in regulating plant fertility, however, they are less studied in wheat. Here, we analyzed lncRNA sequencing data and showed that the fixation carbon sequestration pathway was closely associated with pollen development and fertility conversion in KTM3315A, and eight differentially expressed genes under different fertility conditions were significantly regulated by TCONS_00093333 (designed as TaHTMAR) and transcription factors TaBBX25 and TaOBF1. Among them, TaBBX25 and TaOBF1 were located in the nucleus and expressed in the early stage of fertile anther development. Gene silencing experiments of TaHTMAR showed that TaHTMAR positively regulated the expression of TaBBX25 and TaOBF1 under fertile conditions, thereby reducing male fertility of KTM3315A. This study confirms the effective roles of TaHTMAR, TaBBX25, and TaOBF1 in the regulation of male fertility in wheat and provides a valuable molecular basis for hybrid wheat breeding.
越来越多的研究表明，长非编码 RNA（lncRNA）在调节植物育性方面发挥着重要作用，但在小麦中研究较少。在这里，我们分析了 lncRNA 测序数据，表明固定碳固存途径与 KTM3315A 花粉发育和育性转换密切相关，在不同育性条件下有八个差异表达基因受到 TCONS_00093333（设计为 TaHTMAR）和转录因子 TaBBX25 和 TaOBF1 的显著调控。其中，TaBBX25 和 TaOBF1 位于细胞核中，并在可育花药发育的早期表达。TaHTMAR 的基因沉默实验表明，TaHTMAR 在可育条件下正向调控 TaBBX25 和 TaOBF1 的表达，从而降低 KTM3315A 的雄性育性。本研究证实了 TaHTMAR、TaBBX25 和 TaOBF1 在小麦雄性育性调控中的有效作用，为杂交小麦的培育提供了有价值的分子基础。
