College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Planta. 2024 May 23;260(1):6. doi: 10.1007/s00425-024-04416-z.
TaAGL66, a MADS-box transcription factor highly expressed in fertile anthers of KTM3315A, regulates anther and/or pollen development, as well as male fertility in wheat with Aegilops kotschyi cytoplasm. Male sterility, as a string of sophisticated biological processes in higher plants, is commonly regulated by transcription factors (TFs). Among them, MADS-box TFs are mainly participated in the processes of floral organ formation and pollen development, which are tightly related to male sterility, but they have been little studied in the reproductive development in wheat. In our study, TaAGL66, a gene that was specifically expressed in spikes and highly expressed in fertile anthers, was identified by RNA sequencing and the expression profiles data of these genes, and qRT-PCR analyses, which was localized to the nucleus. Silencing of TaAGL66 under fertility condition in KTM3315A, a thermo-sensitive male sterile line with Ae. kotschyi cytoplasm, displayed severe fertility reduction, abnormal anther dehiscence, defective pollen development, decreased viability, and low seed-setting. It can be concluded that TaAGL66 plays an important role in wheat pollen development in the presence of Ae. kotschyi cytoplasm, providing new insights into the utilization of male sterility.
TaAGL66 是一个在 KTM3315A 可育花药中高度表达的 MADS-box 转录因子，它调节花药和/或花粉发育以及含节节麦细胞质的小麦的雄性育性。雄性不育是高等植物一系列复杂的生物学过程，通常受转录因子（TFs）调控。其中，MADS-box TFs 主要参与花器官形成和花粉发育过程，与雄性不育密切相关，但在小麦生殖发育中的研究较少。在我们的研究中，通过 RNA 测序和这些基因的表达谱数据以及 qRT-PCR 分析，鉴定了一个在穗部特异性表达且在可育花药中高度表达的基因 TaAGL66，它被定位到细胞核中。在含节节麦细胞质的热敏雄性不育系 KTM3315A 的育性条件下沉默 TaAGL66，导致严重的育性降低、花药开裂异常、花粉发育缺陷、活力下降和结实率低。可以得出结论，TaAGL66 在含节节麦细胞质的小麦花粉发育中发挥重要作用，为雄性不育的利用提供了新的见解。
