Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture; Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
J Basic Microbiol. 2022 Nov;62(11):1337-1345. doi: 10.1002/jobm.202200279. Epub 2022 Jul 6.
Trehalose-6-phosphate synthase (TPS) is a key enzyme that participates in trehalose metabolism, which can synthesize trehalose in a two-step pathway with trehalose phosphatase, but its role in fungi is rarely studied, especially in large basidiomycetes. In this study, the tps gene of Ganoderma lucidum was cloned and named as gltps. And gltps-silenced strains were constructed by RNA interference. In this study, it is found that the extracellular polysaccharide content increased 1.6-2-fold, but there was no significant change on intracellular polysaccharide content in gltps-silenced strains compared with the wild-type (WT) strain. Furthermore, the cell wall compositions of the gltps-silenced strains were also altered, which showed that the chitin and β-1,3-glucan contents were significantly decreased. Compared with WT, the concentration of chitin decreased by 20%-50% and that of β-1, 3-glucan decreased by 15%-30%. The study found that the cells of gltps-silenced strains were more sensitive to cell wall stress, which might be due to changes in the compounds and structure of the cell wall. These results showed that gltps had an important effect on carbohydrate metabolism of G. lucidum cells.
海藻糖-6-磷酸合酶（TPS）是参与海藻糖代谢的关键酶，它可以通过海藻糖磷酸酶两步途径合成海藻糖，但它在真菌中的作用很少被研究，特别是在大型担子菌中。在本研究中，克隆了灵芝的 tps 基因，并将其命名为 gltps。并通过 RNA 干扰构建了 gltps 沉默菌株。在本研究中发现，与野生型（WT）菌株相比，gltps 沉默菌株的胞外多糖含量增加了 1.6-2 倍，但胞内多糖含量没有明显变化。此外，gltps 沉默菌株的细胞壁组成也发生了改变，表现为几丁质和β-1,3-葡聚糖含量显著降低。与 WT 相比，几丁质的浓度降低了 20%-50%，β-1,3-葡聚糖的浓度降低了 15%-30%。研究发现，gltps 沉默菌株的细胞对细胞壁应激更敏感，这可能是由于细胞壁化合物和结构的变化所致。这些结果表明 gltps 对灵芝细胞的碳水化合物代谢有重要影响。
