Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
FEBS Open Bio. 2022 Oct;12(10):1782-1787. doi: 10.1002/2211-5463.13463. Epub 2022 Jul 19.
ABCE1 protein (Rli1 in Saccharomyces cerevisiae) is a unique ribosome recycling factor that is composed of an N-terminal FeS cluster domain and two ATPase domains. Here, we report that heterologous expression of human ABCE1 in S. cerevisiae is unable to complement conditional knockout of ABCE1 (Rli1), at a typical experimental temperature of 30 °C. However, low but significant growth was observed at high temperature, 37 °C. Considering the close interaction of ABCE1 with translation factors and ribosomal components, the observed temperature-dependent complementation may be attributed to heterologous co-functionality of ABCE1 with S. cerevisiae factor(s), and might reflect functional upregulation of human ABCE1 at its functional temperature.
ABCE1 蛋白（酿酒酵母中的 Rli1）是一种独特的核糖体回收因子，由一个 N 端 FeS 簇结构域和两个 ATP 酶结构域组成。在这里，我们报告在酿酒酵母中异源表达人 ABCE1 不能在 30°C 的典型实验温度下弥补 ABCE1（Rli1）的条件性缺失。然而，在高温 37°C 时观察到低但显著的生长。考虑到 ABCE1 与翻译因子和核糖体成分的紧密相互作用，观察到的温度依赖性互补可能归因于 ABCE1 与酿酒酵母因子的异源共功能，并且可能反映了人 ABCE1 在其功能温度下的功能上调。
