人 ABCE1 在 S. cerevisiae 中表现出温度依赖性的异源共功能性。
Human ABCE1 exhibits temperature-dependent heterologous co-functionality in S. cerevisiae.
机构信息
Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
出版信息
FEBS Open Bio. 2022 Oct;12(10):1782-1787. doi: 10.1002/2211-5463.13463. Epub 2022 Jul 19.
ABCE1 protein (Rli1 in Saccharomyces cerevisiae) is a unique ribosome recycling factor that is composed of an N-terminal FeS cluster domain and two ATPase domains. Here, we report that heterologous expression of human ABCE1 in S. cerevisiae is unable to complement conditional knockout of ABCE1 (Rli1), at a typical experimental temperature of 30 °C. However, low but significant growth was observed at high temperature, 37 °C. Considering the close interaction of ABCE1 with translation factors and ribosomal components, the observed temperature-dependent complementation may be attributed to heterologous co-functionality of ABCE1 with S. cerevisiae factor(s), and might reflect functional upregulation of human ABCE1 at its functional temperature.
ABCE1 蛋白（酿酒酵母中的 Rli1）是一种独特的核糖体回收因子，由一个 N 端 FeS 簇结构域和两个 ATP 酶结构域组成。在这里，我们报告在酿酒酵母中异源表达人 ABCE1 不能在 30°C 的典型实验温度下弥补 ABCE1（Rli1）的条件性缺失。然而，在高温 37°C 时观察到低但显著的生长。考虑到 ABCE1 与翻译因子和核糖体成分的紧密相互作用，观察到的温度依赖性互补可能归因于 ABCE1 与酿酒酵母因子的异源共功能，并且可能反映了人 ABCE1 在其功能温度下的功能上调。
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