Key Laboratory of the Ministry of Health for Research On Quality and Standardization of Biotech Products, National Institutes of Food and Drug Control, 31 Huatuo Road, Beijing, 102629, People's Republic of China.
Arch Microbiol. 2022 Jul 6;204(8):460. doi: 10.1007/s00203-022-02987-7.
Leptospira interrogans serogroup Icterohaemorrhagiae is the predominant pathogen causing leptospirosis in China and is still used as the vaccine strain for the current human inactivated vaccine. Unlike the clade ST17, which is distributed worldwide, ST1 is the most prevalent in serogroup Icterohaemorrhagiae in China. To further characterize leptospiral pathogens, isobaric tags for relative and absolute quantitation and parallel reaction monitoring were used to analyze differences at the proteomic level between serogroup Icterohaemorrhagiae vaccine strain 56001 (ST1) and circulating isolate 200502 (ST17) from different periods. Two hundred and eighty-one proteins were differentially expressed between the circulating isolate and vaccine strain, of which 166 were upregulated (> 1.2-fold change, P < 0.05) and 115 (< 0.8-fold change, P < 0.05) were downregulated. Function prediction revealed that nine upregulated proteins were outer membrane proteins, including several known immunogenic and/or virulence-related proteins, such as ompL1, LipL71, and LipL41. Furthermore, important expression differences in carbohydrate, amino acid, and energy metabolism and transport proteins were identified between both strains from different clusters, suggesting that these differences may reflect metabolic diversity and the potential of the pathogens to adapt to different environments. In summary, our findings provide insights into a better understanding of the component strains of the Chinese human leptospirosis vaccine at the proteomic level. Additionally, these data facilitate evaluating the mechanisms by which pathogenic Leptospira species adapt to the host environment.
问号钩端螺旋体血清群 Icterohaemorrhagiae 是引起中国钩端螺旋体病的主要病原体，目前仍被用作人类灭活疫苗的疫苗株。与分布于全球的 clade ST17 不同，ST1 是中国血清群 Icterohaemorrhagiae 中最流行的。为了进一步表征钩端螺旋体病原体，采用相对和绝对定量同位素标记（iTRAQ）和平行反应监测（PRM）技术分析了不同时期血清群 Icterohaemorrhagiae 疫苗株 56001（ST1）和流行分离株 200502（ST17）在蛋白质组水平上的差异。在流行株和疫苗株之间有 281 种蛋白质表达差异，其中 166 种上调（>1.2 倍变化，P<0.05），115 种下调（<0.8 倍变化，P<0.05）。功能预测显示，9 种上调的蛋白质是外膜蛋白，包括几个已知的免疫原性和/或毒力相关蛋白，如 ompL1、LipL71 和 LipL41。此外，两个不同聚类的菌株之间的碳水化合物、氨基酸和能量代谢和转运蛋白的重要表达差异表明，这些差异可能反映了代谢多样性和病原体适应不同环境的潜力。总之，我们的研究结果为深入了解中国人类钩端螺旋体疫苗的组成菌株在蛋白质组水平上提供了新的认识。此外，这些数据有助于评估致病性钩端螺旋体适应宿主环境的机制。
