Department of Laboratory Technology, Almus Vocational School, Tokat Gaziosmanpasa University, Tokat, Turkey.
Anal Sci. 2022 Sep;38(9):1207-1212. doi: 10.1007/s44211-022-00146-y. Epub 2022 Jul 6.
In this study, it was aimed to perform the colorimetric determination of nitrite in waters using the redox reaction of iodide in acidic conditions with smartphone. The simple redox reaction of iodide and nitrite in an acidic medium was used instead of the conventional nitrite methods based on the formation of azo dye. The quantitative relationship between the yellow-colored iodine formed because of the reaction and nitrite was determined by the digital image-based colorimetric method (DIC). In the optimal experimental conditions, detection limit and R values were determined as 0.02 mg/L and 0.9925, respectively. New method (Redox-DIC method) was validated by the standard Griess Method for nitrite in lake waters. The perfect recovery results (94-112%) were calculated for lake, river, tap, and spring waters spiked at different concentrations, and very good (< 5.0%) percent relative standard deviation values were recorded. Unlike conventional nitrite methods, organic reagents and solvents were not used in this method. The method is fast, simple, and low cost.
本研究旨在使用智能手机在酸性条件下进行碘化物的氧化还原反应来对水样中的亚硝酸盐进行比色测定。该方法采用简单的碘化物和亚硝酸盐在酸性介质中的氧化还原反应，替代了传统的基于偶氮染料形成的亚硝酸盐方法。通过基于数字图像的比色法（DIC）确定由于反应而形成的黄色碘与亚硝酸盐之间的定量关系。在最佳实验条件下，检测限和 R 值分别确定为 0.02 mg/L 和 0.9925。新方法（氧化还原-DIC 方法）通过标准的 Griess 法对湖泊水中的亚硝酸盐进行了验证。对于在不同浓度下添加的湖泊、河流、自来水和泉水，计算出了完美的回收率（94-112%），并记录了非常好的（<5.0%）相对标准偏差值。与传统的亚硝酸盐方法不同，该方法不使用有机试剂和溶剂。该方法快速、简单且成本低。
