Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Department of Biology, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Department of Biology, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
Cell Rep. 2022 Jul 5;40(1):111049. doi: 10.1016/j.celrep.2022.111049.
The perinuclear theca (PT) is a cytoskeletal element encapsulating the sperm nucleus; however, our understanding of the physiological roles of PT in sperm is very limited. We show that Calicin interacts with itself and many other PT components, indicating it may serve as an organizing center of the PT assembly. Calicin is detectable first when surrounding the acrosome, then detected around the entire nucleus, and finally translocated to the postacrosomal region of spermatid heads. Intriguingly, loss of Calicin specifically causes surface subsidence of sperm heads in the nuclear condensation stage. Calicin interacts with inner acrosomal membrane (IAM) protein Spaca1 and nuclear envelope (NE) components to form an "IAM-PT-NE" structure. Intriguingly, Ccin-knockout sperm also exhibit DNA damage and failure of fertilization. Our study provides solid animal evidence to suggest that the PT encapsulating sperm nucleus helps shape the sperm head and maintain the nuclear structure.
核周质（PT）是包裹精子核的细胞骨架成分；然而，我们对 PT 在精子中的生理作用的理解非常有限。我们表明 Calicin 与自身和许多其他 PT 成分相互作用，表明它可能作为 PT 组装的组织中心。Calicin 首先在顶体周围被检测到，然后在整个核周围被检测到，最后转移到精子头部的顶体后区域。有趣的是，Calicin 的缺失特异性地导致核浓缩阶段精子头部表面下沉。Calicin 与内顶体膜（IAM）蛋白 Spaca1 和核膜（NE）成分相互作用形成“IAM-PT-NE”结构。有趣的是，Calicin 敲除精子也表现出 DNA 损伤和受精失败。我们的研究为 PT 包裹精子核有助于塑造精子头部和维持核结构提供了确凿的动物证据。
