Gifu Prefectural Chuo Livestock Hygiene Service Center, Gifu, Japan.
Joint Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
J Vet Med Sci. 2022 Sep 1;84(9):1157-1163. doi: 10.1292/jvms.22-0206. Epub 2022 Jul 7.
Infectious bronchitis virus (IBV) is the causative agent of infectious bronchitis (IB) in chickens. There is a correlation between cross-protection and percentage of similarity between nucleotide sequences encoding the S1 subunit, which is responsible for generating neutralizing and serotype-specific antibodies. Therefore, RT-PCR is commonly used to amplify the IBV-S1 gene following DNA sequencing in order to predict the efficacy of vaccines against IBV strains. We successfully enhanced the sensitivity for detection of the IBV-S1 gene by second PCR after purification of the 1st RT-PCR product. Using that method, we obtained detailed information on the prevalence of IBV on poultry farms in Gifu Prefecture, Japan. The IBV-S1 gene detection method used in the current study will enable accurate information on the prevalence of IBV in Japan to be obtained.
传染性支气管炎病毒（IBV）是鸡传染性支气管炎（IB）的病原体。S1 亚单位编码核苷酸序列的相似性百分比与交叉保护作用之间存在相关性，S1 亚单位负责产生中和和血清型特异性抗体。因此，通常使用 RT-PCR 对 IBV-S1 基因进行扩增，然后对 DNA 进行测序，以预测 IBV 株疫苗的功效。我们通过在纯化第 1 轮 RT-PCR 产物后进行第二轮 PCR，成功提高了对 IBV-S1 基因的检测灵敏度。使用该方法，我们获得了日本岐阜县家禽养殖场 IBV 流行情况的详细信息。本研究中使用的 IBV-S1 基因检测方法将使日本能够获得关于 IBV 流行情况的准确信息。
