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人类神经母细胞瘤细胞对 SARS-CoV-2 反应的转录组图谱。

Transcriptional landscape of human neuroblastoma cells in response to SARS-CoV-2.

机构信息

State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China.

出版信息

BMC Neurosci. 2022 Jul 6;23(1):43. doi: 10.1186/s12868-022-00728-6.

DOI:10.1186/s12868-022-00728-6
PMID:35794518
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9258770/
Abstract

BACKGROUND

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and the neurological symptoms of SARS-CoV-2 infection have already been reported. However, the mechanisms underlying the effect of SARS-CoV-2 infection on patients with central nervous system injuries remain unclear.

METHODS

The high-throughput RNA sequencing was applied to analyze the transcriptomic changes in SK-N-SH cells after SARS-CoV-2 infection. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to identify the functions of differentially expressed genes and related pathways.

RESULTS

A total of 820 mRNAs were significantly altered, including 671 upregulated and 149 downregulated mRNAs (showing an increase of ≥ 2-fold or decrease to ≤ 0.5-fold, respectively; p ≤ 0.05). Moreover, we verified the significant induction of cytokines, chemokines, and their receptors, as well as the activation of NF-κB, p38, and Akt signaling pathways, in SK-N-SH by SARS-CoV-2.

CONCLUSIONS

To our knowledge, this is the first time the transcriptional profiles of the host mRNAs involved in SARS-CoV-2 infection of SK-N-SH cells have been reported. These findings provide novel insight into the pathogenic mechanism of SARS-CoV-2 and might constitute a new approach for future prevention and treatment of SARS-CoV-2-induced central nervous system infection.

摘要

背景

严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）具有高度传染性，其感染后的神经系统症状已有报道。然而，SARS-CoV-2 感染对中枢神经系统损伤患者的影响机制尚不清楚。

方法

采用高通量 RNA 测序分析 SARS-CoV-2 感染 SK-N-SH 细胞后的转录组变化。进行基因本体论和京都基因与基因组百科全书分析，以鉴定差异表达基因及其相关通路的功能。

结果

共鉴定到 820 个差异表达的 mRNA，包括 671 个上调和 149 个下调的 mRNA（分别表现为增加≥2 倍或减少至≤0.5 倍；p≤0.05）。此外，我们通过 SARS-CoV-2 验证了 SK-N-SH 中细胞因子、趋化因子及其受体的显著诱导，以及 NF-κB、p38 和 Akt 信号通路的激活。

结论

据我们所知，这是首次报道 SARS-CoV-2 感染 SK-N-SH 细胞中涉及的宿主 mRNA 的转录谱。这些发现为 SARS-CoV-2 的致病机制提供了新的见解，可能为未来预防和治疗 SARS-CoV-2 诱导的中枢神经系统感染提供新的方法。

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