Pons Clara, Casals Joan, Palombieri Samuela, Fontanet Lilian, Riccini Alessandro, Rambla Jose Luis, Ruggiero Alessandra, Figás Maria Del Rosario, Plazas Mariola, Koukounaras Athanasios, Picarella Maurizio E, Sulli Maria, Fisher Josef, Ziarsolo Peio, Blanca Jose, Cañizares Joaquin, Cammareri Maria, Vitiello Antonella, Batelli Giorgia, Kanellis Angelos, Brouwer Matthijs, Finkers Richard, Nikoloudis Konstantinos, Soler Salvador, Giuliano Giovanni, Grillo Stephania, Grandillo Silvana, Zamir Dani, Mazzucato Andrea, Causse Mathilde, Díez Maria José, Prohens Jaime, Monforte Antonio Jose, Granell Antonio
Instituto de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València, València, Spain.
Instituto de Biología Molecular y Celular de Plantas (IBMCP). Consejo Superior de Investigaciones Científicas (CSIC), Universitat Politècnica de València, València, Spain.
Hortic Res. 2022 May 17;9:uhac112. doi: 10.1093/hr/uhac112. eCollection 2022.
The Mediterranean basin countries are considered secondary centres of tomato diversification. However, information on phenotypic and allelic variation of local tomato materials is still limited. Here we report on the evaluation of the largest traditional tomato collection, which includes 1499 accessions from Southern Europe. Analyses of 70 traits revealed a broad range of phenotypic variability with different distributions among countries, with the culinary end use within each country being the main driver of tomato diversification. Furthermore, eight main tomato types (phenoclusters) were defined by integrating phenotypic data, country of origin, and end use. Genome-wide association study (GWAS) meta-analyses identified associations in 211 loci, 159 of which were novel. The multidimensional integration of phenoclusters and the GWAS meta-analysis identified the molecular signatures for each traditional tomato type and indicated that signatures originated from differential combinations of loci, which in some cases converged in the same tomato phenotype. Our results provide a roadmap for studying and exploiting this untapped tomato diversity.
地中海盆地国家被视为番茄多样化的次级中心。然而，关于当地番茄材料的表型和等位基因变异的信息仍然有限。在此，我们报告了对最大的传统番茄种质库的评估，该种质库包含来自南欧的1499份种质。对70个性状的分析揭示了广泛的表型变异性，不同国家之间分布各异，每个国家内的烹饪最终用途是番茄多样化的主要驱动因素。此外，通过整合表型数据、原产国和最终用途，定义了八种主要的番茄类型（表型簇）。全基因组关联研究（GWAS）荟萃分析在211个位点鉴定出关联，其中159个是新发现的。表型簇与GWAS荟萃分析的多维整合确定了每种传统番茄类型的分子特征，并表明这些特征源自位点的不同组合，在某些情况下这些组合在相同的番茄表型中汇聚。我们的结果为研究和利用这种未开发的番茄多样性提供了路线图。
