Research Center of Environmental Pollutants, Qom University of Medical Sciences, Qom, Iran.
Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences Tehran, Tehran, Iran.
Cutan Ocul Toxicol. 2022 Sep;41(3):243-249. doi: 10.1080/15569527.2022.2096628. Epub 2022 Jul 21.
As a major public health issue, skin cancer is a leading reason of death and has resulted in significant financial and human losses globally. Numerous environmental and internal variables may both drive and exacerbate the pathophysiology of skin cancer. Marine herbs and animals, including marine sponges, cucumbers, and squirts, have been shown to have cytotoxic consequences on cancerous cells in prior research.
melanoma mitochondria obtained from the skin of melanoma animal models are studied in this research to see whether extracts from , a plant endemic to the northern coasts of the Persian Gulf, have a cytotoxic impact on them.
In this study, the mitochondria were isolated from melanoma cells via differential centrifugation and treated with various concentrations (1250, 2500 and 5000 µg/ml) of methanolic extract of Then MTT, ROS, MMP decline, mitochondrial swelling, cytochrome c release and flow cytometry assays were performed on
The results of the MTT assay showed that the IC50 of extract is 2500 μg/ml and extract induced a selectively significant ( < 0.05) concentration-dependent decrease in the SDH activity in cancerous skin mitochondria. The ROS results also showed that all concentrations of extracts significantly increased ROS production, MMP decline and the release of cytochrome c in cancer group mitochondria. The swelling of mitochondria isolated from the cancer group was significantly increased compared to the control group. In addition, the results of the apoptosis assay showed that the addition of root extract of on melanoma cells increased apoptosis, while it had no effect on control non-tumour cells.
Based on these results, the presence of potentially bioactive compounds in makes this Persian Gulf coastal herb a strong candidate for further molecular studies and clinical research in the field of melanoma cancer therapy.
皮肤癌作为一个主要的公共卫生问题，是全球范围内导致死亡和重大经济及人力损失的主要原因。众多环境和内部因素都可能导致和加剧皮肤癌的病理生理学变化。先前的研究表明，海洋植物和动物，包括海绵、黄瓜和海鞘，对癌细胞具有细胞毒性作用。
本研究旨在探讨来自黑色素瘤动物模型皮肤的黑色素瘤线粒体，是否会受到来自波斯湾北部沿海特有植物 的提取物的影响。
在这项研究中，通过差速离心法从黑色素瘤细胞中分离出线粒体，并使用不同浓度（1250、2500 和 5000μg/ml）的 甲醇提取物进行处理。然后进行 MTT、ROS、MMP 下降、线粒体肿胀、细胞色素 c 释放和流式细胞术检测。
MTT 检测结果表明， 提取物的 IC50 为 2500μg/ml， 提取物诱导癌细胞线粒体中 SDH 活性呈浓度依赖性显著下降（ < 0.05）。ROS 结果还表明， 提取物的所有浓度均能显著增加 ROS 产生、MMP 下降和细胞色素 c 的释放，而癌症组线粒体的肿胀程度明显高于对照组。此外，凋亡检测结果表明， 根提取物的加入可增加黑色素瘤细胞的凋亡，而对非肿瘤对照细胞无影响。
基于这些结果， 波斯湾沿海这种草药中可能存在具有潜在生物活性的化合物，使其成为黑色素瘤癌症治疗领域进一步分子研究和临床研究的有力候选者。
