Selective toxicity of root extract on cancerous skin mitochondria isolated from animal model of melanoma.

INTRODUCTION

As a major public health issue, skin cancer is a leading reason of death and has resulted in significant financial and human losses globally. Numerous environmental and internal variables may both drive and exacerbate the pathophysiology of skin cancer. Marine herbs and animals, including marine sponges, cucumbers, and squirts, have been shown to have cytotoxic consequences on cancerous cells in prior research.

PURPOSE

melanoma mitochondria obtained from the skin of melanoma animal models are studied in this research to see whether extracts from , a plant endemic to the northern coasts of the Persian Gulf, have a cytotoxic impact on them.

MATERIAL AND METHOD

In this study, the mitochondria were isolated from melanoma cells via differential centrifugation and treated with various concentrations (1250, 2500 and 5000 µg/ml) of methanolic extract of Then MTT, ROS, MMP decline, mitochondrial swelling, cytochrome c release and flow cytometry assays were performed on

RESULTS

The results of the MTT assay showed that the IC50 of extract is 2500 μg/ml and extract induced a selectively significant ( < 0.05) concentration-dependent decrease in the SDH activity in cancerous skin mitochondria. The ROS results also showed that all concentrations of extracts significantly increased ROS production, MMP decline and the release of cytochrome c in cancer group mitochondria. The swelling of mitochondria isolated from the cancer group was significantly increased compared to the control group. In addition, the results of the apoptosis assay showed that the addition of root extract of on melanoma cells increased apoptosis, while it had no effect on control non-tumour cells.

DISCUSSION AND CONCLUSION

Based on these results, the presence of potentially bioactive compounds in makes this Persian Gulf coastal herb a strong candidate for further molecular studies and clinical research in the field of melanoma cancer therapy.