Departmant of Infectious Diseases & Clinical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, 34093, Turkey.
Department of Infectious Diseases & Clinical Microbiology, Haseki Training & Research Hospital, Istanbul, 34096, Turkey.
Future Microbiol. 2022 Sep;17:1027-1042. doi: 10.2217/fmb-2021-0305. Epub 2022 Jul 7.
The authors aimed to determine the efficacy of frequently used antibiotics, alone or in combination, against biofilms of ventilator-associated pneumonia isolates. The authors determined the MICs, minimum biofilm inhibitory concentrations and minimum biofilm eradication concentrations of meropenem, ciprofloxacin and colistin as well as their combinations against planktonic forms and biofilms of , and clinical isolates. Generally, the minimum biofilm inhibitory concentrations and minimum biofilm eradication concentrations of the antibiotics were 1000-fold higher than their MICs, and synergy was provided by different concentrations of meropenem-colistin and meropenem-ciprofloxacin combinations with checkerboard and time-kill curve methods. The combination of meropenem and ciprofloxacin seems to be a good candidate for the treatment of biofilm-associated infections; none of the concentrations obtained as a result of the synergy test were clinically significant.
作者旨在确定常用抗生素单独或联合使用对抗呼吸机相关性肺炎分离株生物膜的疗效。作者测定了美罗培南、环丙沙星和黏菌素单独及联合应用对 、 、 临床分离株浮游生物和生物膜的 MIC、最小生物膜抑制浓度和最小生物膜清除浓度。通常，抗生素的最小生物膜抑制浓度和最小生物膜清除浓度是 MIC 的 1000 倍，美罗培南-黏菌素和美罗培南-环丙沙星联合应用的棋盘法和时间杀伤曲线法可提供协同作用。美罗培南和环丙沙星联合似乎是治疗生物膜相关感染的一个很好的选择；协同试验获得的浓度均无临床意义。
