MD. Physician, Assistant Professor, Department of Infectious Diseases and Clinical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.
PhD. Professor, Department Microbiology and Clinical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Sao Paulo Med J. 2024 Feb 23;142(4):e2023113. doi: 10.1590/1516-3180.2023.0113.R1.29112023. eCollection 2024.
To the best of our knowledge, this is the first study to evaluate the effectiveness of specific concentrations of antibiofilm agents, such as N-acetyl cysteine (NAC), rifampicin, and ozone, for the treatment of pan-resistant Klebsiella pneumoniae (PRKp).
We evaluated the effectiveness of antibiofilm agents, such as NAC, rifampicin, and ozone, on biofilm formation in PRKp at 2, 6, 24, and 72 h.
This single-center experimental study was conducted on June 15, 2017, and July 15, 2018, at Istanbul Faculty of Medicine, Istanbul University, Turkey.
Biofilm formation and the efficacy of these agents on the biofilm layer were demonstrated using colony counting and laser-screened confocal microscopy.
NAC at a final concentration of 2 μg/mL was administered to bacteria that formed biofilms (24 h), and no significant decrease was detected in the bacterial counts of all isolates (all P > 0.05). Rifampicin with a final concentration of 0.1 μg/mL was administered to bacteria that formed biofilm (24 h), and no significant decrease was detected in bacterial count (all P > 0.05). Notably, ozonated water of even 4.78 mg/L concentration for 72 h decreased the bacterial count by ≥ 2 log10.
Different approaches are needed for treating PRKp isolates. We demonstrate that PRKp isolates can be successfully treated with higher concentrations of ozone.
据我们所知，这是第一项评估特定浓度抗生物膜剂（如 N-乙酰半胱氨酸[NAC]、利福平、臭氧）治疗泛耐药肺炎克雷伯菌（PRKp）效果的研究。
我们评估了 NAC、利福平、臭氧等抗生物膜剂在 2、6、24 和 72 小时时对 PRKp 生物膜形成的影响。
这是一项于 2017 年 6 月 15 日和 2018 年 7 月 15 日在土耳其伊斯坦布尔大学伊斯坦布尔医学院进行的单中心实验研究。
使用菌落计数和激光筛选共聚焦显微镜来展示生物膜形成和这些药物对生物膜层的效果。
将终浓度为 2μg/mL 的 NAC 施用于形成生物膜的细菌（24 小时），但所有分离株的细菌计数均未显著下降（均 P＞0.05）。将终浓度为 0.1μg/mL 的利福平施用于形成生物膜的细菌（24 小时），但细菌计数无明显下降（均 P＞0.05）。值得注意的是，浓度为 4.78mg/L 的臭氧水甚至作用 72 小时后，细菌计数减少了≥2log10。
需要针对治疗 PRKp 分离株采取不同的方法。我们证明可以使用较高浓度的臭氧成功治疗 PRKp 分离株。
