College of Pharmacy, Xinxiang Medical University, Xinxiang, P.R. China.
Sanquan College, Xinxiang Medical University, Xinxiang, P.R. China.
J Biomater Sci Polym Ed. 2022 Dec;33(17):2185-2201. doi: 10.1080/09205063.2022.2099664. Epub 2022 Jul 19.
In this study, novel redox-sensitive nanoparticles (NPs) were fabricated from the poly(caprolactone) conjugates with disulfide-linked poly(ethylene glycol) (DDMAT- mPEG-S-S-PCL, DPSP). The DPSP polymer was synthesized by ring-opening polymerization (ROP) and reversible addition-fragmentation chain transfer (RAFT) polymerization. The obtaining of the DPSP polymer was confirmed by the H nuclear magnetic resonance (H NMR) and Fourier transform infrared spectroscopy (FTIR) spectra. The DPSP NPs were fabricated with the solvent-evaporation method. Docetaxel (DTX) was employed as a model drug and encapsulated into the DPSP NPs. The anti-tumor activity of the DTX-loaded DPSP NPs and free DTX against the breast cancer cells (4T1) were evaluated by MTT assay. The cargo-free DPSP NPs were in circular shapes with an average diameter of 107.8 ± 0.4 nm. These NPs displayed redox-responsive behavior in the presence of glutathione. Animal experiments indicated that the DPSP NPs showed excellent blood compatibility and good bio-security. Cell tests suggested that the DPSP NPs could be taken in by 4T1 cells, smoothly, which improved the anti-tumor activity of free DTX.
在这项研究中，新型氧化还原敏感纳米粒子（NPs）是由带有二硫键连接的聚乙二醇（DDMAT-mPEG-S-S-PCL，DPSP）的聚己内酯（PCL）缀合物制备的。DPSP 聚合物是通过开环聚合（ROP）和可逆加成-断裂链转移（RAFT）聚合合成的。通过 H 核磁共振（H NMR）和傅里叶变换红外光谱（FTIR）谱证实了 DPSP 聚合物的获得。通过溶剂挥发法制备了 DPSP NPs。多西他赛（DTX）被用作模型药物并包封到 DPSP NPs 中。通过 MTT 测定法评估了负载 DTX 的 DPSP NPs 和游离 DTX 对乳腺癌细胞（4T1）的抗肿瘤活性。无载药的 DPSP NPs 呈圆形，平均直径为 107.8±0.4nm。这些 NPs 在谷胱甘肽存在下表现出氧化还原响应行为。动物实验表明，DPSP NPs 具有良好的血液相容性和良好的生物安全性。细胞试验表明，DPSP NPs 可以被 4T1 细胞摄取，从而提高游离 DTX 的抗肿瘤活性。
