Wang Xiaojie, Dai Yarui, Zhang Jialu, Li Xin
Chengde Medical University, Chengde, 067000, Hebei, The People's Republic of China.
Arch Gynecol Obstet. 2023 Mar;307(3):937-943. doi: 10.1007/s00404-022-06524-1. Epub 2022 Jul 7.
Cervical cancer is a common malignant gynecological disease that threatens the health of women all over the world. The abnormal expression of Annexin A5 (ANXA5) is closely related to the biological behavior of various malignant tumors, however, the relationship between ANXA5 and cervical cancer is still unclear. Therefore, the effects of low expression of ANXA5 on the proliferation, apoptosis, migration and invasion of cervical cancer cells (HeLa) and its related mechanism were explored.
The cells were divided into three groups: ANXA5-si group, negative control group and blank group. RNA interference was used to suppress ANXA5 expression. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony formation assay, flow cytometry and propidium iodide (PI) staining, wound healing assay and transwell assay were employed to detect cell proliferation, apoptosis, migration and invasion respectively. Meanwhile, gene expression was detected by qPCR and Western blotting.
ANXA5 suppression lead to the increase of proliferation, migration, invasion and the decrease of apoptosis of cervical cancer HeLa cells. Furthermore, the expression of both pPI3K and pAkt increased.
ANXA5 might inhibit Hela cells proliferation and metastasis by regulating PI3K/Akt signal pathway.
宫颈癌是一种常见的妇科恶性疾病，威胁着全球女性的健康。膜联蛋白A5（ANXA5）的异常表达与各种恶性肿瘤的生物学行为密切相关，然而，ANXA5与宫颈癌之间的关系仍不清楚。因此，探讨了ANXA5低表达对宫颈癌细胞（HeLa）增殖、凋亡、迁移和侵袭的影响及其相关机制。
将细胞分为三组：ANXA5-si组、阴性对照组和空白组。采用RNA干扰抑制ANXA5表达。分别采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法、集落形成试验、流式细胞术和碘化丙啶（PI）染色、伤口愈合试验和Transwell试验检测细胞增殖、凋亡、迁移和侵袭。同时，通过qPCR和蛋白质印迹法检测基因表达。
抑制ANXA5导致宫颈癌HeLa细胞增殖、迁移、侵袭增加，凋亡减少。此外，pPI3K和pAkt的表达均增加。
ANXA5可能通过调节PI3K/Akt信号通路抑制HeLa细胞增殖和转移。
