Human annexin A5 promotes glioma progression by targeting the pathway.

BACKGROUND

Gliomas are the most common intracranial malignant tumors. In this study, we aimed to identify the hub genes and investigate the pathophysiological significance of in glioma.

METHODS

The differentially expressed genes (DEGs) between tumor and adjacent tissues from glioma patients were acquired from the Gene Expression Omnibus (GEO) database. Functional enrichment analysis and protein-protein interaction (PPI) network construction of overlapping DEGs were performed. The GEPIA and CGGA databases were used to explore hub gene expression. The effect of hub genes on prognosis and tumor-infiltrating immune cells was analyzed via GEPIA, CGGA, and TIMER2 databases. Additionally, expression was measured by qRT-PCR and Western blotting. The effects of were assessed by CCK-8, colony formation, Transwell, and flow cytometry assays. Moreover, the roles of were identified .

RESULTS

The DEGs were enriched in cell surface receptor signaling pathway, immune response, and signaling pathway. The selected hub genes were included , , , , , and . Among them, expression of , , , , and was strongly correlated with patient prognosis and was also involved in the tumor microenvironment. Furthermore, knockdown significantly inhibited the migration and proliferation of glioma cells and . Meanwhile, we found that the expression of was monitored by , and promoted the migration and proliferation of glioma cells via the pathway.

CONCLUSION

Taken together, our data showed that could contribute to cell proliferation and metastasis of glioma by targeting the axis.