Aquaculture and Sea-Ranching, University Rostock, Rostock, Germany.
Faculty of Fisheries and Marine Sciences, IPB Bogor Agricultural University, Bogor, Indonesia.
Acta Parasitol. 2022 Sep;67(3):1307-1328. doi: 10.1007/s11686-022-00581-x. Epub 2022 Jul 7.
The most convincing species of Allopodocotyle Pritchard, 1966 (Digenea: Opecoelidae) are known overwhelmingly from groupers (Serranidae: Epinephelinae). Six species of Allopodocotyle have been reported, collectively, from species of Cromileptes Swainson, 1839, Epinephelus Bloch, 1793 and Plectropomus Oken, 1817. These are A. epinepheli (Yamaguti, 1942), A. heronensis Downie & Cribb, 2011, A. manteri (Saoud & Ramadan, 1984), A. mecopera (Manter, 1940), A. plectropomi (Manter, 1963) and A. serrani (Yamaguti, 1952). In addition, a not yet fully described and unnamed seventh species, morphologically and phylogenetically close to A. epinepheli, was isolated from the orange-spotted grouper Epinephelus coioides (Hamilton, 1822) off Bali, Indonesia in 2016. An eighth species, again from E. coioides off Bali is described herein.
Morphological and phylogenetic analyses justify the recognition of A. palmi sp. nov., which is also genetically different from the as yet unnamed congener from the same host and locality. For the first time, 3D confocal laser scanning microscopy was applied to study and distinguish Digenea taxonomically. We introduce the 'Palm pattern', a new simplified way to visualise morphometric differences of related digenean taxa.
Allopodocotyle palmi sp. nov. is distinguished from its congeners that infect groupers by its elongate body with a size > 2.7 mm and diagonal testes. The ovary is located mainly, and the anterior testis completely, in the posterior half of the body; the uterine coils are in the fourth eighth of the body. The cirrus-sac is 0.75-1.4 (1.1) mm long, its posterior extremity is well separated from the anterior extent of the vitelline fields, just reaching the anterior border of uterine coils. In addition, Prosorhynchus maternus Bray & Justine, 2006 (Bucephalidae) was isolated from E. coioides, representing the first record in Indonesia and the third record for this fish species.
The biodiversity research in Indonesia is enhanced with a new species description based on modern and newly applied techniques.
最令人信服的 Allopodocotyle Pritchard, 1966 种（吸虫纲：Opecoelidae）绝大多数来自石斑鱼（Serranidae: Epinephelinae）。已经报道了六种 Allopodocotyle 物种，它们共同来自 Cromileptes Swainson, 1839、Epinephelus Bloch, 1793 和 Plectropomus Oken, 1817 种。这些是 A. epinepheli (Yamaguti, 1942)、A. heronensis Downie & Cribb, 2011、A. manteri (Saoud & Ramadan, 1984)、A. mecopera (Manter, 1940)、A. plectropomi (Manter, 1963) 和 A. serrani (Yamaguti, 1952)。此外，2016 年在印度尼西亚巴厘岛从橙色斑点石斑鱼 Epinephelus coioides (Hamilton, 1822) 中分离出一种尚未完全描述和命名的第七种物种，形态学和系统发育上与 A. epinepheli 密切相关。本文描述了第八种来自巴厘岛的同种石斑鱼的物种。
形态学和系统发育分析证明了 A. palmi sp. nov. 的存在，该物种与同一宿主和同一地点尚未命名的同种在遗传上也不同。本文首次应用三维共聚焦激光扫描显微镜对吸虫进行分类研究。我们引入了“棕榈图案”，这是一种新的简化方法，可以可视化相关吸虫分类群的形态差异。
Allopodocotyle palmi sp. nov. 与感染石斑鱼的同属种不同，其特征为体长大于 2.7 毫米和斜形睾丸。卵巢主要位于身体后半部，前睾丸完全位于后半部；子宫圈位于身体的第四到第八段。阴茎囊长 0.75-1.4（1.1）毫米，其后端与卵黄区的前端完全分开，仅到达子宫圈的前缘。此外，从 E. coioides 中分离出 Prosorhynchus maternus Bray & Justine, 2006（Bucephalidae），这是印度尼西亚的首次记录，也是该鱼类的第三次记录。
基于现代和新应用技术的新物种描述增强了印度尼西亚的生物多样性研究。
