基于 UPA-Seq 的功能 lncRNA 候选物搜索方法。

UPA-Seq-Based Search Method for Functional lncRNA Candidates.

机构信息

RNA Biology Laboratory, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

Global Station for Biosurfaces and Drug Discovery, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan.

出版信息

Methods Mol Biol. 2022;2509:269-278. doi: 10.1007/978-1-0716-2380-0_16.

DOI:10.1007/978-1-0716-2380-0_16

PMID:35796969

Abstract

Long noncoding RNAs (lncRNAs) constitute a large fraction of the transcriptome in mammals, and recent studies have revealed important functions of lncRNAs in a variety of biological processes. However, the fraction of lncRNAs that have been functionally validated is small, and only sequence and expression information are available for most lncRNAs. Here, we describe the procedures for UV-phenol aqueous-phase RNA sequencing (UPA-seq), a method for searching for functional lncRNA candidates among whole genomes based on the assumption that functional lncRNAs exert their functions through associations with proteins.

摘要

长链非编码 RNA（lncRNA）构成了哺乳动物转录组的很大一部分，最近的研究揭示了 lncRNA 在多种生物过程中的重要功能。然而，经过功能验证的 lncRNA 比例很小，而且大多数 lncRNA 只有序列和表达信息。在这里，我们描述了 UV-酚水相 RNA 测序（UPA-seq）的程序，这是一种基于功能 lncRNA 通过与蛋白质结合发挥作用的假设，在全基因组范围内搜索功能 lncRNA 候选物的方法。