Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association, Osaka, Japan.
Laboratory of Environmental Toxicology and Carcinogenesis, School of Pharmacy, Nihon University, Chiba, Japan.
J Occup Health. 2022 Jan;64(1):e12345. doi: 10.1002/1348-9585.12345.
We aimed to develop a method to determine workers' personal exposure levels to N-(phosphonomethyl)glycine (glyphosate) for their risk assessments.
The proposed method was assessed as follows: recovery, stability of samples on storage, method limit of quantification, and reproducibility. Glyphosate in air was sampled using an air-sampling cassette containing a glass fiber filter. Ultrapure water was used to extract glyphosate from sampler filters. After derivation with 9-fluorenylmethyloxycarbonyl chloride, samples were analyzed by high-performance liquid chromatography using a fluorescence detector.
Spiked samples indicated an overall recovery of 101%. After 7 days of storage at 4°C, recoveries were approximately 100%. The method limit of quantification was 0.060 μg/sample. Relative standard deviations representing overall reproducibility, defined as precision, were 1.4%-1.8%.
The method developed in this study allows 4-h personal exposure monitoring of glyphosate at 0.250-500 μg/m . Thus, this method can be used to estimate worker exposure to glyphosate.
我们旨在开发一种方法来确定工人接触 N-(膦酸甲基)甘氨酸（草甘膦）的个人暴露水平，以进行风险评估。
评估了以下提出的方法：样品的回收率、储存时的稳定性、方法定量限和重现性。使用含有玻璃纤维过滤器的空气采样盒采集空气中的草甘膦。超纯水用于从采样器过滤器中提取草甘膦。用 9-芴甲氧羰基氯衍生后，通过荧光检测器使用高效液相色谱法对样品进行分析。
加标样品的总回收率为 101%。在 4°C 下储存 7 天后，回收率约为 100%。方法定量限为 0.060μg/样品。代表整体重现性（定义为精密度）的相对标准偏差为 1.4%-1.8%。
本研究中开发的方法允许在 0.250-500μg/m 3的情况下进行 4 小时个人草甘膦暴露监测。因此，该方法可用于估计工人接触草甘膦的情况。
