Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Biomedical Engineering Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
J Wound Care. 2022 Jul 2;31(7):598-611. doi: 10.12968/jowc.2022.31.7.598.
The present study aims to create -loaded nanofibre-based wound dressing materials to enhance the wound healing process. is an annual herb native to the Mediterranean region. It is antipyretic, antifungal, antioedema, antidiabetic, anti-inflammatory (wound, oral and pharyngeal mucosa), antispasmodic, treats chronic ocular surface diseases, acts as a stimulant and a diaphoretic. It is also used in the prevention of acute dermatitis, and in the treatment of gastrointestinal ulcers, wounds and burns.
Electrospinning is an effective method for creating nano- and microfibres for biomedical applications. (CA) of various concentrations 5%, 10% and 15%)-loaded polyvinyl alcohol (PVA)/sodium alginate (SAlg) nanofibre mats were successfully produced via blend electrospinning. Nanofibre mats were evaluated using: scanning electron microscopy (SEM); Fourier transform infrared spectroscopy (FTIR) analysis; gel content; water vapour transmission rate (WVTR); swelling ratio; in vitro drug release studies; viability evaluation (cell culture and MTT assay); and an in vivo study using male Wistar rats. Rats were divided into three groups (n=3). In each group, rats were inflicted with five full-thickness wounds on the back and were treated with sterile gauze (control), PVA/SAlg nanofibre dressing (CA-free control), PVA/SAlg/CA5%, PVA/SAlg/CA10%, and PVA/SAlg/CA15% nanofibre dressing.
Results showed that the obtained fibres were smooth with no surface aggregates, indicating complete incorporation of . The release of from loaded PVA/SAlg fibre mats in the first four hours was burst released and then was constant. PVA/SAlg and PVA/SAlg/CA nanofibres were not toxic to L929 mouse fibroblasts and supported cell attachment and proliferation. The results of the in vivo study showed that the PVA/SAlg/CA10% nanofibre dressing had a higher full-thickness wound healing closure rate compared with the control group on days seven, 14 and 21 after treatment.
The results of this evaluation showed that PVA/SAlg/CA nanofibrous mats could be a candidate as an effective wound dressing; however, the percentage of CA in this compound needs further investigation.
本研究旨在制备负载 的纳米纤维创面修复材料，以促进创面愈合过程。 是一种原产于地中海地区的一年生草本植物。它具有解热、抗真菌、抗水肿、降血糖、抗炎（口腔和咽黏膜）、抗痉挛、治疗慢性眼表面疾病、作为兴奋剂和发汗剂的作用。它还用于预防急性皮炎，以及治疗胃肠道溃疡、伤口和烧伤。
静电纺丝是一种用于生物医学应用的制备纳米和微纤维的有效方法。 通过共混静电纺丝成功制备了负载 的聚醋酸乙烯酯（PVA）/海藻酸钠（SAlg）纳米纤维垫，其中 （CA）的浓度分别为 5%、10%和 15%。使用扫描电子显微镜（SEM）；傅里叶变换红外光谱（FTIR）分析；凝胶含量；水蒸气透过率（WVTR）；溶胀比；体外药物释放研究；细胞活力评估（细胞培养和 MTT 测定）；以及雄性 Wistar 大鼠的体内研究来评估纳米纤维垫。大鼠分为三组（n=3）。在每组中，大鼠背部有五个全层伤口，用无菌纱布（对照组）、PVA/SAlg 纳米纤维敷料（无 CA 对照组）、PVA/SAlg/CA5%、PVA/SAlg/CA10%和 PVA/SAlg/CA15%纳米纤维敷料进行处理。
结果表明，获得的纤维光滑无表面团聚，表明 完全掺入。负载 PVA/SAlg 纤维垫在最初的四个小时内 释放是突释的，然后是恒定的。PVA/SAlg 和 PVA/SAlg/CA 纳米纤维对 L929 小鼠成纤维细胞无毒，支持细胞附着和增殖。体内研究结果表明，与对照组相比，PVA/SAlg/CA10%纳米纤维敷料在治疗后第 7、14 和 21 天具有更高的全层伤口愈合闭合率。
评估结果表明，PVA/SAlg/CA 纳米纤维垫可以作为一种有效的伤口敷料候选物；然而，该化合物中 CA 的百分比需要进一步研究。
