Warton A, Phillips M J, Papadimitriou J M
Aust J Exp Biol Med Sci. 1986 Oct;64 ( Pt 5):419-34. doi: 10.1038/icb.1986.45.
Pulmonary macrophages from normal subjects and asthmatic patients were examined for the presence of sugar residues on their surface. The technique of bronchoalveolar lavage was employed to obtain cell samples. Ultrastructural and cytophotometric methods were used for studying the patterns of lectin binding by these two groups of macrophages. Three lectins, Concanavalin A (Con A), Wheat germ agglutinin (WGA) and Ricinus communis agglutinin (RCA), were used in this investigation. Pulmonary macrophages from both normal and asthmatic persons revealed a high level of Con A, WGA and RCA binding. The distribution of the electron dense reaction product on the macrophage surfaces was relatively uniform. Quantitative cytophotometric studies showed that the level of binding of Con A by macrophages from both groups was approximately the same. Similar results were obtained with WGA--the difference between macrophages from normal and asthmatic persons was not statistically significant. In the case of RCA, macrophages from asthmatic patients showed a higher level of lectin binding than macrophages from normal persons. The conclusion is made that macrophages from asthmatic persons have more D-galactose residues on their surface.
对正常人和哮喘患者的肺巨噬细胞表面的糖残基进行了检测。采用支气管肺泡灌洗技术获取细胞样本。利用超微结构和细胞光度测定法研究这两组巨噬细胞的凝集素结合模式。本研究使用了三种凝集素，即刀豆球蛋白A（Con A）、麦胚凝集素（WGA）和蓖麻凝集素（RCA）。正常人和哮喘患者的肺巨噬细胞均显示出高水平的Con A、WGA和RCA结合。巨噬细胞表面电子致密反应产物的分布相对均匀。定量细胞光度测定研究表明，两组巨噬细胞对Con A的结合水平大致相同。WGA也得到了类似结果——正常人和哮喘患者巨噬细胞之间的差异无统计学意义。就RCA而言，哮喘患者的巨噬细胞比正常人的巨噬细胞显示出更高水平的凝集素结合。得出的结论是，哮喘患者的巨噬细胞表面有更多的D-半乳糖残基。
