Shen Yanting, Xin Zhichuan, Hu Xiaoxiao, Wang Nan, Liu Shuxia, Wang Jing
Postdoctoral Mobile Station of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, People's Republic of China; School of Pharmacy, Key Laboratory of Innovative Drug Development and Evaluation, Hebei Medical University, Shijiazhuang 050017, People's Republic of China.
School of Pharmacy, Key Laboratory of Innovative Drug Development and Evaluation, Hebei Medical University, Shijiazhuang 050017, People's Republic of China.
Talanta. 2022 Dec 1;250:123712. doi: 10.1016/j.talanta.2022.123712. Epub 2022 Jul 3.
As one kind of reactive oxygen species, hydrogen peroxide (HO) participated in various cellular biological processes including cell differentiation and inflammation responses. Abnormal HO level is closely related to cancer and other diseases. Highly sensitive detection and monitoring HO are of great importance for understanding the roles of HO in cellular dynamic events. Herein, a novel dual stimulus-responsive core-satellite surface-enhanced Raman scattering (SERS) nanoprobe engineered with manganese dioxide (MnO) and silver nanoparticles (Ag NPs) was constructed for sensitive HO detection. The sensing strategy is based on the target-triggered degradation both of the "core" and "satellite". In this system, the MnO core not only could be used as solid supporter to generate "hot spots" that can induce strong SERS signals, but also acted as the responsive unit for HO sensing together with Ag NPs. A good linear relationship in the range from 1 to 100 μM and limit of detection of 7.44 μM were obtained. Moreover, the nanosensor possessed good repeatability. Based on this strategy, the sensitive detection of cellular HO was achieved. Furthermore, the SERS-based HO monitoring during the starvation-induced autophagy was realized by the developed nanoprobes. Our study provides a new way for sensitive HO detection and opens a new avenue for sensing and detection of other biomolecules.
作为一种活性氧，过氧化氢（HO）参与了包括细胞分化和炎症反应在内的各种细胞生物学过程。HO水平异常与癌症和其他疾病密切相关。对HO进行高度灵敏的检测和监测对于理解HO在细胞动态事件中的作用至关重要。在此，构建了一种新型的由二氧化锰（MnO）和银纳米颗粒（Ag NPs）设计的双刺激响应核-卫星表面增强拉曼散射（SERS）纳米探针，用于灵敏检测HO。传感策略基于“核”和“卫星”的目标触发降解。在该系统中，MnO核不仅可作为产生能诱导强SERS信号的“热点”的固体载体，还与Ag NPs一起作为HO传感的响应单元。获得了1至100μM范围内良好的线性关系以及7.44μM的检测限。此外，该纳米传感器具有良好的重复性。基于此策略，实现了对细胞内HO的灵敏检测。此外，通过所开发的纳米探针实现了饥饿诱导自噬过程中基于SERS的HO监测。我们的研究为灵敏检测HO提供了一种新方法，并为其他生物分子的传感和检测开辟了一条新途径。
