School of Pharmaceutical Sciences, Key Laboratory of Innovative Drug Development and Evaluation, Hebei Medical University, Shijiazhuang, 050017, People's Republic of China.
Mikrochim Acta. 2023 Aug 2;190(8):341. doi: 10.1007/s00604-023-05870-4.
A novel stimulus-responsive surface-enhanced Raman scattering (SERS) nanoprobe has been developed for sensitive glutathione (GSH) detection based on manganese dioxide (MnO) core and silver/gold nanoparticles (Ag/Au NPs). The MnO core is not only capable to act as a scaffold to amplify the SERS signal via producing "hot spots", but also can be degraded in the presence of the target and thus greatly enhance the nanoprobe sensitivity for sensing of GSH. This approach enables a wide linear range from 1 to 100 µM with a 2.95 µM (3σ/m) detection limit. Moreover, the developed SERS nanoprobe represents great possibility in both sensitive detection of intracellular GSH and even can monitor the change of intracellular GSH level when the stimulant occurs. This sensing system not merely offers a novel strategy for sensitive sensing of GSH, but also provides a new avenue for other biomolecules detection.
一种新型的刺激响应表面增强拉曼散射（SERS）纳米探针已被开发出来，用于基于二氧化锰（MnO）核和银/金纳米粒子（Ag/Au NPs）的灵敏谷胱甘肽（GSH）检测。MnO 核不仅能够作为通过产生“热点”来放大 SERS 信号的支架，而且还可以在存在目标的情况下被降解，从而大大提高了纳米探针对 GSH 感应的灵敏度。这种方法能够实现从 1 到 100 μM 的宽线性范围，检测限为 2.95 μM（3σ/m）。此外，所开发的 SERS 纳米探针在灵敏检测细胞内 GSH 方面具有很大的可能性，甚至可以在刺激发生时监测细胞内 GSH 水平的变化。该传感系统不仅为 GSH 的灵敏检测提供了一种新策略，也为其他生物分子的检测提供了新途径。
