Department of Biology, Hong Kong Baptist University, Hong Kong.
Department of Biology, Hong Kong Baptist University, Hong Kong; Institute of Bioresources and Agriculture and Sino-Forest Applied Research Centre for Pearl River Delta Environment, Hong Kong Baptist University, Hong Kong.
Bioresour Technol. 2022 Sep;360:127578. doi: 10.1016/j.biortech.2022.127578. Epub 2022 Jul 4.
Reaching industrially relevant productivities in bioprocesses and their efficient integration in the existing industrial infrastructure remain as important challenges in the circular economy to create closed loop sustainability framework. Using anaerobic digestion (AD) biorefinery as a model, the present work addressed these problems via integration of next-generation rhamnolipids production with AD. A high rhamnolipids concentration of 10.25 ± 1.34 g/L was obtained by fed-batch fermentation using food waste digestate as medium. Digestate-derived rhamnolipids contained Rha-C-C and Rha-Rha-C-C as the predominant congeners. These were used back in single-phase AD to demonstrate their effect on sludge solubilization and digestion efficiency. A dosage of 0.02 g rhamnolipids/g total suspended solids was found to be optimal which enhanced the hydrolysis-acidogenesis reactions to up to 27% over control. It however retarded methane production which could be overcome by the prolongation of digestion time. Finally, the value chain appreciation by the proposed process was demonstrated by a feasibility analysis.
在循环经济中，要创建闭环可持续性框架，实现生物工艺的工业化相关生产力和将其高效整合到现有工业基础设施中仍然是重要的挑战。本工作以厌氧消化（AD）生物炼制为模型，通过将下一代鼠李糖脂生产与 AD 整合来解决这些问题。使用食物废物消化物作为培养基进行分批补料发酵，可获得 10.25±1.34 g/L 的高鼠李糖脂浓度。消化物衍生的鼠李糖脂含有 Rha-C-C 和 Rha-Rha-C-C 作为主要同系物。这些同系物被重新用于单相 AD 中，以证明它们对污泥溶解和消化效率的影响。发现 0.02 g 鼠李糖脂/g 总悬浮固体的剂量是最佳的，可将水解-产酸反应提高至对照的 27%。然而，它会延迟甲烷的产生，这可以通过延长消化时间来克服。最后，通过可行性分析证明了所提出的工艺的价值链增值。
