Department of Biomolecular Physics, Faculty of Physics, Babes-Bolyai University, M Kogalniceanu Str. 1, 400084 Cluj-Napoca, Romania; Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute in Bio-Nano-Sciences, Babes-Bolyai University, T. Laurian Str. 42, 400271 Cluj-Napoca, Romania.
Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute in Bio-Nano-Sciences, Babes-Bolyai University, T. Laurian Str. 42, 400271 Cluj-Napoca, Romania.
Carbohydr Polym. 2022 Oct 1;293:119736. doi: 10.1016/j.carbpol.2022.119736. Epub 2022 Jun 16.
We describe a mild, ecofriendly, and straightforward two-step strategy for making core-shell Au@Ag bimetallic nanoparticles (BMNPs) for antibacterial nanomedicine and SERS imaging. The synthesis exploits the unique properties of the cationic polymeric cyclodextrin (PolyCD) as both reducing and stabilizing agent to obtain, monodispersed and stable Au@Ag BMNPs. PolyCD-driven protocol includes the synthesis of PolyCD-coated Au monometallic nanoparticles (MNPs) as a seed material for the subsequent growing of a silver shell. PolyCD was produced by polymerization of the azido modified βCD monomers with epichlorohydrin and subsequent reduction of azido derivative. The amino groups, as hydrochloride salts (one for CD ring), are pivotal for the formation of BMNPs in mild conditions. Nanoantibiotics and SERS-nanoTag were prepared by complexation of Au@Ag BMNPs with Linezolid (Lz) and 4-mercaptophenyl boronic acid, respectively. Au@Ag@Lz complexes showed a good antibacterial activity against all tested microorganisms including the methicillin resistant Staphylococcus aureus (MRSA).
我们描述了一种温和、环保、直接的两步策略，用于制造用于抗菌纳米医学和 SERS 成像的核壳 Au@Ag 双金属纳米粒子（BMNPs）。该合成利用阳离子聚合物环糊精（PolyCD）的独特性质作为还原剂和稳定剂，以获得单分散且稳定的 Au@Ag BMNPs。PolyCD 驱动的方案包括合成 PolyCD 包覆的 Au 单金属纳米粒子（MNPs）作为随后生长银壳的种子材料。PolyCD 是通过将叠氮改性的 βCD 单体与环氧氯丙烷聚合，然后还原叠氮衍生物而制备的。氨基，作为盐酸盐（一个用于 CD 环），对于在温和条件下形成 BMNPs 至关重要。纳米抗生素和 SERS-nanoTag 分别通过 Au@Ag BMNPs 与利奈唑胺（Lz）和 4-巯基苯硼酸的络合制备。Au@Ag@Lz 配合物对所有测试的微生物（包括耐甲氧西林金黄色葡萄球菌（MRSA））均显示出良好的抗菌活性。
