用于钙调蛋白和抗钙调蛋白的超微量酶联免疫吸附测定
Ultramicro-ELISA for calmodulin and anticalmodulin.
作者信息
Wenz I, Reissmann R, Börnig H, Hoppe H, Cumme G
出版信息
Biomed Biochim Acta. 1987;46(1):125-33.
A non-competitive ELISA-technique for the determination of anticalmodulin as well as a sensitive competitive ELISA for calmodulin in 10 microliters samples has been developed. The assay for calmodulin has an inter-assay and intra-assay variability of 18% and 17%, respectively; the limit of detection is 20 pg per assay. The corresponding values for the assay of anticalmodulin are less than 6%, less than 6% and 35 pg, respectively. The methods for the purification of calmodulin and the production of anticalmodulin are also described.
已开发出一种用于测定抗钙调蛋白的非竞争性酶联免疫吸附测定（ELISA）技术以及一种用于在10微升样品中检测钙调蛋白的灵敏竞争性ELISA。钙调蛋白测定的批间和批内变异系数分别为18%和17%；检测限为每次测定20皮克。抗钙调蛋白测定的相应值分别小于6%、小于6%和35皮克。还描述了钙调蛋白的纯化方法和抗钙调蛋白的制备方法。
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